A supplement‐free osteoclast–osteoblast co‐culture for pre‐clinical application

Schulze Sabine; Wehrum Diana; Dieter PeterHempel Ute

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A supplement‐free osteoclast–osteoblast co‐culture for pre‐clinical application

机译：A增刊‐free osteoclast-osteoblast co‐朔提前临床应用

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There is increasing demand for efficient and physiological in vitro cell culture systems suitable for testing new pharmaceutical drugs or for evaluating materials for tissue regeneration. In particular, co‐cultures of two or more tissue‐relevant cell types have the advantage to study the response of cells on diverse parameters in a more natural environment with respect to physiological complexity. We developed a direct bone cell co‐culture system using human peripheral blood monocytes (hPBMC) and human bone marrow stromal cells (hBMSC) as osteoclast/osteoblast precursor cells, respectively, strictly avoiding external supplements for the induction of differentiation. The sophisticated direct hPBMC/hBMSC co‐culture was characterized focusing on osteoclast function and was compared with two indirect approaches. Only in the direct co‐culture, hPBMC were triggered by hBMSC into osteoclastogenesis and became active resorbing osteoclasts. Bisphosphonates and sulfated glycosaminoglycans were used to examine the suitability of the co‐culture system for evaluating the influence of certain effectors on bone healing and bone regeneration and the contribution of each cell type thereby. The results show that the investigated substances had more pronounced effects on both osteoblasts and osteoclasts in the co‐culture system than in respective monocultures.

机译：对高效、有越来越多的需求生理体外细胞培养系统适用于测试新的药物或评估组织再生的材料。特别是,公司的两个或两个以上的文化组织相关的细胞类型有优势研究细胞的反应不同的参数对在一个更自然的环境生理的复杂性。骨细胞有限公司使用人类文化系统外周血单核细胞(hPBMC)和人类的骨头骨髓基质细胞(hBMSC)破骨细胞和成骨细胞前体细胞,分别,严格避免外部补充剂的诱导分化。的直接hPBMC / hBMSC公司文化特点是集中于破骨细胞功能的,而两种间接的方法。只有在直接公司特色文化,hPBMC由hBMSC osteoclastogenesis和触发突起成为活跃的破骨细胞。磷酸盐和硫酸粘多糖被用来研究的适用性公司文化系统评估的影响某些效应器在骨愈合和骨每个细胞的再生和贡献从而类型。研究物质更加明显对成骨细胞和破骨细胞的影响公司检测比各自的文化体系单一栽培。

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来源
《Journal of Cellular Physiology》 |2018年第6期|4391-4400|共10页
作者
Schulze Sabine; Wehrum Diana; Dieter PeterHempel Ute;
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Faculty of Medicine Carl Gustav CarusTechnische Universit?t DresdenDresden,Germany;

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正文语种英语
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关键词
Osteoclast; Osteoclastogenesis; tissue regenerationperipheral blood mononuclear cellGlycosaminoglycansOsteoblasttissue repairDiphosphonatesCell Culture SystemBone Marrow Stromal Cellsbone healingNatural environmentcell type;

机译：regenerationperipheral血液单核细胞repairDiphosphonatesCell文化SystemBone骨髓基质Cellsbone healingNaturalenvironmentcell类型;

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A supplement‐free osteoclast–osteoblast co‐culture for pre‐clinical application

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