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首页> 外文期刊>Journal of Cellular Physiology >Proteomic expression profile of injured rat peripheral nerves revealed biological networks and processes associated with nerve regeneration
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Proteomic expression profile of injured rat peripheral nerves revealed biological networks and processes associated with nerve regeneration

机译：蛋白质组表达谱的受伤的老鼠周围神经生物网络和过程与神经再生有关

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摘要

Peripheral nerve regeneration is regulated through the coordinated spatio‐temporal activation of multiple cellular pathways. In this work, an integrated proteomics and bioinformatics approach was employed to identify differentially expressed proteins at the injury‐site of rat sciatic nerve at 20 days after damage. By a label‐free liquid chromatography mass‐spectrometry (LC‐MS/MS) approach, we identified 201 differentially proteins that were assigned to specific canonical and disease and function pathways. These include proteins involved in cytoskeleton signaling and remodeling, acute phase response, and cellular metabolism. Metabolic proteins were significantly modulated after nerve injury to support a specific metabolic demand. In particular, we identified a group of proteins involved in lipid uptake and lipid storage metabolism. Immunofluorescent staining for acyl‐CoA diacylglycerol acyltransferase 1 (DGAT1) and DAGT2 expression provided evidence for the expression and localization of these two isoforms in Schwann cells at the injury site in the sciatic nerve. This further supports a specific local regulation of lipid metabolism in peripheral nerve after damage.
机译：周围神经再生是通过监管协调spatio时间激活多个细胞通路。整合蛋白质组学和生物信息学方法是用来识别差异表达蛋白在大鼠坐骨神经损伤所致的地点在损伤后20天。色谱质谱应承担的地理(LC / MS)女士的方法,我们发现201不同蛋白质被分配到特定的规范和疾病和功能通路。参与细胞骨架蛋白的信号装修,急性期反应,细胞新陈代谢。调节神经损伤后支持特定的代谢需求。确定一组蛋白质参与脂质吸收和脂质存储新陈代谢。Immunofluorescent染色为酰基辅酶a二酰基甘油酰基转移酶1 (DGAT1)和DAGT2表达式提供了证据这两个亚型的表达和定位雪旺细胞在损伤部位坐骨神经。局部调节脂质代谢后周围神经损害。

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