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首页> 外文期刊>Journal of Cellular Physiology >The small GTPase RhoA regulates the LIMK1/2‐cofilin pathway to modulate cytoskeletal dynamics in oocyte meiosis
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The small GTPase RhoA regulates the LIMK1/2‐cofilin pathway to modulate cytoskeletal dynamics in oocyte meiosis

机译：小GTPase RhoA调节的LIMK1/2 cofilin途径调节细胞骨架动力学在卵母细胞减数分裂

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摘要

LIM kinases (LIMK1/2) are LIM domain‐containing serine/threonine/tyrosine kinases that mediate multiple cellular processes in mitosis. In the present study, we explored the functional roles and potential signaling pathway of LIMK1/2 during mouse oocyte meiosis. Disruption of LIMK1/2 activity and expression significantly decreased oocyte polar body extrusion. Live‐cell imaging revealed that spindle migration was disturbed after both LIMK1 and LIMK2 knock down, and this might be due to aberrant distribution of actin filaments in the oocyte cytoplasm and cortex. Meanwhile, our results demonstrated that the function of LIMK1 and LIMK2 in actin assembly was related to cofilin phosphorylation levels. In addition, disruption of LIMK1/2 activity significantly increased the percentage of oocytes with abnormal spindle morphologies, which was confirmed by the abnormal p‐MAPK localization. We further, explored the upstream molecules of LIMK1/2, and we found that after depletion of ROCK, phosphorylation of LIMK1/2 and cofilin were significantly decreased. Moreover, RhoA inhibition caused the decreased expression of ROCK, p‐LIMK1/2, and cofilin. In summary, our results indicated that the small GTPase RhoA regulated LIMK1/2‐cofilin to modulate cytoskeletal dynamics during mouse oocyte meiosis.
机译：LIM激酶(LIMK1/2) LIM域包含丝氨酸/苏氨酸/酪氨酸激酶,调解多个细胞在有丝分裂过程。目前的研究中,我们探讨了功能角色和潜在的LIMK1/2的信号通路小鼠卵母细胞减数分裂。活动和表达显著下降卵母细胞极体挤压。透露,主轴迁移干扰后LIMK1和LIMK2击倒,这可能是由于异常的肌动蛋白的分布丝在卵母细胞细胞质和皮层。与此同时,我们的研究结果表明函数LIMK1和LIMK2肌动蛋白组装相关cofilin磷酸化水平。此外,LIMK1/2活动的破坏显著提高卵母细胞的百分比与纺锤体异常形态,确认,异常p MAPK本地化。进一步探讨了上游的分子LIMK1/2,耗尽后我们发现岩石,LIMK1/2和cofilin的磷酸化大大降低了。抑制导致减少的表达岩石,p必经LIMK1/2, cofilin。结果表明,小GTPase RhoA监管LIMK1/2 cofilin应承担的调节在小鼠卵母细胞细胞骨架动力学减数分裂。

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