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首页> 外文期刊>Journal of Cellular Physiology >Increased N6‐methyladenosine causes infertility is associated with FTO expression
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Increased N6‐methyladenosine causes infertility is associated with FTO expression

机译：N6 methyladenosine应承担的增加会导致不孕FTO表达相关的

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摘要

The N6‐methyladenosine (m6A) modification plays a central role in epigenetic regulation of the mammalian transcriptome. m6A can be demethylated by the fat mass‐ and obesity‐associated (FTO) protein and the α‐ketoglutarate‐dependent dioxygenase alkB homolog 5 (ALKBH5) protein. Much less is known about that whether m6A content is involved in POI (premature ovarian insufficiency) disease. In this case‐controlled study, 69 POI and 53 tubal occlusion patients were recruited from the reproduction centers in our hospital. For the POI animal model experiment, ovarian tissue was obtained from ten POI and nine healthy mice. An m6A test kit was developed to determine the m6A content in the RNA, and qPCR and western blot were used to examine the mRNA and protein expression levels of FTO and ALKBH5. FACS was used to measure the levels of proliferation and apoptosis, and siRNA was used to establish FTO and ALKBH5 knockdown cell lines. Our results showed that the m6A content in the RNA from POI patients and POI mice was significantly higher than control groups and that POI was characterized by the content of m6A. The mRNA and protein expression levels of FTO were significantly lower in the POI patients than control group and were associated with a risk of POI. These data suggest that the decreased mRNA and protein expression levels of FTO may be responsible for the increase in m6A in POI, which may further increase the risk of complications of POI. High m6A should be investigated further as a novel potential biomarker of POI.
机译：N6 methyladenosine应承担(m6A)修改中表观遗传调控的核心作用哺乳动物的转录组。相关的脂肪量和肥胖所致(FTO)蛋白质和α酮戊二酸应承担的依赖加双氧酶alkB同族体5 (ALKBH5)的蛋白质。所知甚少,是否m6A内容参与POI(过早卵巢机能不全)疾病。53输卵管阻塞患者招募在我们医院生殖中心。POI的动物模型实验中,卵巢组织了从十POI和9健康老鼠。在RNA m6A内容,qPCR和西方污点是用来检查信使rna和蛋白质表达水平的FTO和ALKBH5。用来测量扩散和的水平细胞凋亡,核是用于建立FTO和ALKBH5击倒细胞系。表明,RNA的POI m6A内容病人和POI老鼠明显更高比对照组和POI特点是m6A的内容。FTO的蛋白表达水平POI显著降低的病人比对照组和相关的风险芋泥。FTO可能和蛋白质表达水平POI负责m6A的增加,可能会进一步增加并发症的风险芋泥。小说POI的潜在生物标志物。

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