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首页> 外文期刊>Journal of Cellular Physiology >Modulation of Mammary Stromal Cell Lactate Dynamics by Ambient Glucose and Epithelial Factors
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Modulation of Mammary Stromal Cell Lactate Dynamics by Ambient Glucose and Epithelial Factors

机译：调制的乳腺间质细胞乳酸动力学环境葡萄糖和上皮因素

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摘要

Hyperglycemia is a risk factor for a variety of human cancers. Increased access to glucose and that tumor metabolize glucose by a glycolytic process even in the presence of oxygen (Warburg effect), provide a framework to analyze a particular set of metabolic adaptation mechanisms that may explain this phenomenon. In the present work, using a mammary stromal cell line derived from healthy tissue that was subjected to a long-term culture in low (5 mM) or high (25 mM) glucose, we analyzed kinetic parameters of lactate transport using a FRET biosensor. Our results indicate that the glucose pre-culture and soluble epithelial factors constitute a stimulus for lactate stromal production, factors that also modify the kinetic parameters and the monocarboxylate transporters expression in stromal cells. We also observed a vectorial flux of lactate from stroma to epithelial cells in a co-culture setting and found that the uptake of lactate by epithelial cells correlates with the degree of malignancy. Glucose preconditioning of the stromal cell stimulated epithelial motility. Our findings suggest that lactate generated by stromal cells in the high glucose condition stimulate epithelial migration. Overall, our results support the notion that glucose not only provides a substrate for tumor nutrition but also behaves as a signal promoting malignancy. (C) 2016 Wiley Periodicals, Inc.
机译：高血糖症是多种危险因素人类癌症。肿瘤代谢葡萄糖的糖酵解甚至在氧气的存在(华宝过程效果),提供一个分析的框架特定的代谢适应机制这或许可以解释这一现象。工作,使用乳基质细胞系受到来自健康组织长期的文化低(5毫米)或高(25毫米)葡萄糖,我们分析了动力学参数乳酸交通用生物传感器。结果表明,葡萄糖pre-culture和溶性上皮构成的刺激因素乳酸基质生产,也因素动力学参数和修改monocarboxylate转运蛋白表达基质细胞。基质中上皮细胞的乳酸培养设置和发现的乳酸通过上皮细胞相关程度的恶性肿瘤。基质细胞刺激上皮细胞的能动性。我们的研究结果表明,乳酸生成的基质细胞在高葡萄糖条件刺激上皮细胞迁移。结果支持了这种观点,即葡萄糖不仅为肿瘤提供了基质营养也行为视为促进恶性肿瘤信号。2016年威利期刊、公司。

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