...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>EMBO Journal >Transcriptional activation by bidirectional RNA polymerase II elongation over a silent promoter
【24h】

Transcriptional activation by bidirectional RNA polymerase II elongation over a silent promoter

机译：通过双向RNA转录激活聚合酶II伸长沉默的启动子

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Transcriptional interference denotes negative cis effects between promoters. Here, we show that promoters can also interact positively. Bidirectional RNA polymerase II (Pol II) elongation over the silent human endogenous retrovirus (HERV)-K 18 promoter (representative of 2.5 x 10(3) similar promoters genomewide) activates transcription. In tandem constructs, an upstream promoter activates HERV-K 18 transcription. This is abolished by inversion of the upstream promoter, or by insertion of a poly(A) signal between the promoters; transcription is restored by poly(A) signal mutants. TATA-box mutants in the upstream promoter reduce HERV-K 18 transcription. Experiments with the same promoters in a convergent orientation produce similar effects. A small promoter deletion partially restores HERV-K 18 activity, consistent with activation resulting from repressor repulsion by the elongating Pol II. Transcriptional elongation over this class of intragenic promoters will generate co-regulated sense-antisense transcripts, or, alternatively initiating transcripts, thus expanding the diversity and complexity of the human transcriptome.
机译：转录干扰表示- cis启动子之间的影响。启动子也可以积极互动。双向RNA聚合酶II (Pol II)沉默的人类内源性伸长逆转录病毒(HERV) - k 18启动子(代表2.5 x 10(3)类似的启动子全基因组)激活转录。上游启动子激活HERV-K 18转录。上游启动子或插入的保利(A)启动子之间的信号;转录是由聚(A)恢复信号突变体。启动子减少HERV-K 18转录。实验与相同的推动者收敛方向产生类似的效果。小的启动子缺失部分恢复HERV-K18日活动,与激活结果一致从延伸波尔阻遏排斥2基因内发起人将生成粘住sense-antisense成绩单,或者,或者开始记录,从而扩大人类的多样性和复杂性转录组。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号