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首页> 外文期刊>Shock: Molecular, cellular, and systemic pathobiological aspects and therapeutic approaches = The official journal of the Shock Society, the European Shock Society, the Brazilian Shock Society, the International Federation of Shock Societies >miR-194 Promotes burn-induced hyperglycemia via attenuating IGF-IR expression.
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miR-194 Promotes burn-induced hyperglycemia via attenuating IGF-IR expression.

机译：mir - 194促进burn-induced高血糖通过衰减IGF-IR表达式。

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Hyperglycemia is one of the most important clinical features of burn patients. Previous reports had demonstrated that miRNA was involved in regulating glucose metabolism in various diseases such as diabetes and obesity. Our current study discovered the relationship between miR-194 and hyperglycemia in burn rats via suppressing insulin-like growth factor 1 receptor (IGF-IR). We found that the fasting blood glucose was significantly increased in rats of the burn group, and protein expression of IGF-IR was attenuated in response to burn injury. Similar to the results of animal experiments, miR-194 expression was significantly elevated and IGF-IR protein level was suppressed in L6 cells treated with serum from burn rats compared with those treated by serum from sham rats. However, IGF-IR mRNA level was comparable between burn and sham rats, suggesting that IGF-IR may be downregulated at the translation level. Further experiments revealed that miR-194 was significantly increased in burn rats compared with sham rats using miRNA array and real-time polymerase chain reaction (PCR) assay. And IGF-IR protein expression was reduced in L6 cells transfected with miR-194 plasmid. Insulin-like growth factor 1 receptor expression was also repressed and fasting blood glucose was increased in rats injected with miR-194 plasmid. In general, we have identified a novel function of miR-194 in modulating burn-induced hyperglycemia via suppressing the expression of IGF-IR.
机译：高血糖是最重要的之一烧伤患者的临床特征。报道表明,microrna的参与在各种调节葡萄糖代谢糖尿病和肥胖等疾病。本研究发现之间的关系mir - 194和高血糖大鼠通过燃烧抑制胰岛素样生长因子1受体(IGF-IR)。显著增加了燃烧的老鼠吗组和蛋白质的表达IGF-IR减毒的反应燃烧伤害。动物实验的结果,mir - 194表达显著升高,IGF-IR蛋白质水平被抑制在16种细胞治疗从烧伤大鼠血清相比从虚假的大鼠血清治疗。信使rna水平相当燃烧与骗局老鼠,这表明IGF-IR可能下调在翻译水平。透露,mir - 194显著增加在烧伤大鼠相比,使用microrna的虚假的老鼠数组和实时聚合酶链反应(PCR)分析。减少社会应激细胞转染mir - 194质粒。表达式也压抑和空腹血在老鼠注射葡萄糖增加mir - 194质粒。小说在调制mir - 194的功能通过抑制burn-induced高血糖IGF-IR的表情。

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