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首页> 外文期刊>Nanoscale >Two-color dark-field (TCDF) microscopy for metal nanoparticle imaging inside cells
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Two-color dark-field (TCDF) microscopy for metal nanoparticle imaging inside cells

机译：金属的双色暗视野显微镜(TCDF)纳米颗粒细胞内成像

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摘要

Noble metal nanoparticles (NPs) supporting localized surface plasmon resonances are widely used in the context of biotechnology as optical and absorption contrast agents with great potential applicability to both diagnostics and less invasive therapies. In this framework, it is crucial to have access to simple and reliable microscopy techniques to monitor the NPs that have internalized into cells. While dark field (DF) microscopy takes advantage of the enhanced NP scattering at their plasmon resonance, its use in cells is limited by the large scattering background from the internal cell compartments. Here, we report on a novel two-color dark field microscopy that addresses these limitations by significantly reducing the cell scattering contribution. We first present the technique and demonstrate its enhanced contrast, specificity and reliability for NP detection compared to a standard optical dark field. We then demonstrate its potential suitability in two different settings, namely wide-field parallel screening of circulating cells in micro-fluidic chips and high-resolution tracking of internalized NPs in cells. These proof of principle experiments show a promising capability of this approach with possible extension to other kinds of targeted systems like bacteria and vesicles.
机译：贵金属纳米颗粒(NPs)支持局部表面等离子体共振是广泛的用于生物技术的背景光和吸收造影剂与伟大潜在的诊断和适用性微创疗法。获得简单而可靠的关键显微技术监督的NPs已经内化成细胞。(DF)显微镜利用增强的NP等离子体共振散射,它的使用在细胞受到大的散射背景从内部细胞的隔间。这里,我们报告一个小说的双色暗视野显微镜,地址这些限制大大减少细胞散射的贡献。展示其增强对比度,特异性为NP和可靠性检测相比标准的光暗视野。其潜在的在两个不同的适用性设置,即广角平行筛选micro-fluidic细胞芯片和传播高分辨率内化NPs的跟踪细胞。一个有前途的这种方法的能力可能扩展到其他类型的目标系统,如细菌和囊泡。

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