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首页> 外文期刊>Ophthalmologica: International Journal of Ophthalmology=Journal International d'Ophtalmologie >The survival of donor-derived cells in a successfully grafted corneal button 10 years after penetrating keratoplasty for lattice dystrophy.
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The survival of donor-derived cells in a successfully grafted corneal button 10 years after penetrating keratoplasty for lattice dystrophy.

机译：donor-derived细胞的生存十年成功移植角膜按钮在穿透角膜移植术晶格营养不良。

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摘要

AIMS: To investigate the survival of donor-derived cells in a successfully grafted corneal button 10 years after penetrating keratoplasty for lattice dystrophy. METHODS: In 1996, a 48-year-old male with lattice corneal dystrophy underwent penetrating keratoplasty 3 times in the right eye within a 3-month interval. Nine years and 7 months later, the patient underwent a fourth penetrating keratoplasty. After surgery, the previous graft was analyzed to determine the origin of the cells. The epithelium and endothelium were removed, and then the button was dissected into 5 stromal blocks measuring 2 x 1.8 mm. Tissues underwent forensic genotyping using 16 markers (amelogenin for sex chromosomes and 15 autosomal short tandem repeats). Patient buccal tissue DNA was used as a control. RESULTS: The epithelium and buccal tissue contained identical DNA (i.e. recipient DNA). Similarly, the most peripheral stromal tissue contained only recipient DNA. In contrast, the most central stromal tissue only contained DNA of nonrecipient origin (presumably donor), while the stromal tissue between the periphery and center contained both recipient and nonrecipient DNA. CONCLUSIONS: The corneal stroma was infiltrated by surrounding recipient-derived keratocytes from the periphery. Therefore, more donor-derived cells had survived in the central stroma.
机译：目的:探讨donor-derived的生存细胞成功地移植角膜按钮10年后穿透角膜移植术晶格营养不良。与晶格角膜营养不良了穿透角膜移植术右眼的3倍在三个月的时间间隔。个月后,病人接受了第四穿透性角膜移植术。以前的移植进行了分析确定细胞的起源。内皮细胞被移除,然后按钮切割成5基质块测量2 x 1.8毫米。组织接受法医基因分型16标记(amelogenin性染色体和15常染色体短串联重复序列)。组织DNA被用作控制。上皮和颊组织包含相同的DNA(即受体DNA)。周围间质组织只包含收件人的DNA。间质组织只包含nonrecipient的DNA起源(大概捐赠),而基质组织之间的边缘和中心控制收件人和nonrecipient DNA。角膜基质被周围的渗透从外围recipient-derived可见。因此,更多donor-derived细胞已经活了下来在中央基质。

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