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首页> 外文期刊>Biology of Reproduction: Offical Journal of the Society for the Study of Reproduction >Functional analysis of male mouse haploid germ cells of various differentiation stages: early and late round spermatids are functionally equivalent in producing progeny.
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Functional analysis of male mouse haploid germ cells of various differentiation stages: early and late round spermatids are functionally equivalent in producing progeny.

机译：男性鼠标单倍体生殖的功能分析细胞不同分化阶段:早和末轮精子功能相当于在产生后代。

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摘要

Spermiogenesis is a complex process consisting of three main phases: the round, elongating, and elongated spermatid phases. Although the germ cells acquire a haploid set of paternal chromosomes after meiosis, how functional these male haploid germ cells are as male gametes at various differentiation stages has remained unclear. We selectively injected specific steps of haploid male germ cells into oocytes and assessed the function of the zygotes. Applying the transillumination technique using acrosin-green fluorescent protein transgenic mice, we succeeded in selecting four types of haploid male germ cells for microinsemination: early round spermatids (steps 2-3), late round spermatids (steps 7-8), elongating spermatids (steps 9-10), and elongated spermatids (step 16). The microinsemination technique revealed that the early and late round spermatids had similar developmental abilities in producing progeny, indicating that the nuclear status of newly generated round spermatids was similar to that of lateround spermatids. An increased birthrate of progeny was first observed in steps 9-10 of elongating spermatids, but the frequency was slightly lower than that of the elongated spermatids. These results indicated that the transition from steps 7-8 of round spermatids to steps 9-10 of elongating spermatids is a key step in changing the nuclear status of male gametes in producing progeny.
机译：精子形成是一个复杂的过程组成的三个主要阶段:圆、延伸和细长的精细胞阶段。细胞获得单倍体的父亲染色体在减数分裂后,这些功能如何男性单倍体生殖细胞男性配子不同分化阶段一直保持不清楚。单倍体的男性生殖细胞在卵母细胞评估了受精卵的函数。透照技术使用acrosin-green荧光蛋白转基因老鼠,我们成功地选择了四种类型的单倍体microinsemination男性生殖细胞:早期精子(步骤2),晚期精子(步骤7 - 8),延伸精子(步骤9 - 10),细长的精子(步骤16)。microinsemination技术显示早期和晚期精子有相似发展能力生产后代,显示最新的核地位生成圆形精子是相似的lateround精子。后代是第一步骤9中观察到延伸精子,但频率略低于拉长精子。从步骤7 - 8轮的精子步骤9延伸的精子是一个关键的步骤在改变男性配子的核地位产生后代。

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