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首页> 外文期刊>Biology of Reproduction: Offical Journal of the Society for the Study of Reproduction >Role of importin alpha8, a new member of the importin alpha family of nuclear transport proteins, in early embryonic development in cattle.
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Role of importin alpha8, a new member of the importin alpha family of nuclear transport proteins, in early embryonic development in cattle.

机译：的角色importin alpha8,一个新成员importinα核运输的家庭蛋白质,在早期胚胎发育牛。

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摘要

Nuclear proteins such as transcription and chromatin remodeling factors are required for initiation of transcription in early embryos before embryonic genome activation. The nuclear transport of these proteins is mediated by transport factors such as importins. Through analysis of expressed sequence tags from a bovine oocyte cDNA library, we identified a new member of the importin alpha family (named importin alpha8). The cloned cDNA for bovine importin alpha8 (KPNA7) is 1817 base pair in length, encoding a protein of 522 amino acids that contains a conserved importin beta-binding domain and seven armadillo motifs. The RT-PCR analysis revealed that KPNA7 mRNA is specifically expressed in ovaries and mature oocytes. Real-time PCR demonstrated that KPNA7 expression in germinal vesicle (GV) oocytes is 33 to 2396 times higher than that of other importin alpha genes and that KPNA7 mRNA is abundant in GV and metaphase II oocytes, as well as in early-stage embryos collected before embryonic genome activation, but is barely detectable in morula- and blastocyst-stage embryos. Similarly, expression of KPNA7 protein is very high in oocytes and early embryos but is low in blastocysts. A glutathione S-transferase pull-down assay revealed that KPNA7 has a strong binding affinity for the nuclear protein nucleoplasmin 2 relative to that of other importin alphas. RNA interference experiments demonstrated that knockdown of KPNA7 in early embryos results in a decreased proportion of embryos developing to the blastocyst stage. These results suggest that KPNA7 may have an important role in the transport of essential nuclear proteins required for early embryogenesis.
机译：核如转录和蛋白质染色质重塑因素所需在早期胚胎的转录的起始在胚胎基因组激活。这些蛋白是由运输运输importins等因素。从牛的分析表达序列标签卵母细胞cDNA文库,我们发现了一个新成员importinα家庭(名为输入蛋白α的alpha8)。alpha8 (KPNA7)是1817个碱基对长度,编码522个氨基酸的蛋白包含一个守恒importin beta-binding域和七个犰狳图案。透露,KPNA7信使rna是特别的表示在卵巢和成熟的卵母细胞。实时PCR表明KPNA7表达式在胚泡(问)卵母细胞是33 - 2396倍比其他importinαKPNA7信使rna基因和在全球之声和丰富在早期中期ⅱ卵母细胞,以及胚胎之前收集胚胎基因组难以觉察的激活,但在桑椹胚和胚泡期胚胎。表达KPNA7蛋白质是非常高的卵母细胞和早期胚胎,但低胚泡。下拉试验表明KPNA7有很强核蛋白质绑定关联相对于其他nucleoplasmin 2importin阿尔法。表明,月初KPNA7击倒胚胎结果的比例下降胚胎囊胚阶段发展。结果表明,KPNA7可能很重要在运输中的作用重要的核早期胚胎发育所需的蛋白质。

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