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首页> 外文期刊>Applied in vitro toxicology. >Profibrotic Activity of Multiwalled Carbon Nanotubes Upon Prolonged Exposures in Different Human Lung Cell Types
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Profibrotic Activity of Multiwalled Carbon Nanotubes Upon Prolonged Exposures in Different Human Lung Cell Types

机译：微碳Profibrotic活动纳米管在长期暴露在不同人类肺细胞类型

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Introduction: The expanding use of multiwalled carbon nanotubes (MWCNTs) in various consumer and industrial products has raised concerns regarding the potential health risks following the inhalation of this nanomaterial. Recent studies indicate that MWCNT exposure can cause deleterious lung effects. Pulmonary fibrosis is one of the diseases associated with the inhalation of classical fiber-shaped materials (e.g., asbestos), and recently MWCNTs. Materials and Methods: Herein, the presented work investigated early signs of fibrosis in key human lung cell types implicated directly (fibroblasts) or indirectly (alveolar epithelial cells and macrophages) in the pathogenesis of lung fibrosis, upon acute (24 hours) and prolonged (96 hours) exposure to different concentrations (5–20 μg/mL) of long, rigid Mitsui-7 MWCNTs. These results were compared to those inflicted upon the same cell types following exposure to tangled nanofibers, that is, Nanocyl-7000, and a positive particle control, crystalline quartz (i.e., Min-U-Sil). Results: Mitsui-7 interacted with all cell types tested, in particular, single nanotubes were readily internalized by each cell type. Exposure to Mitsui-7 resulted in a proinflammatory response in macrophages at 24 hours (interleukin-1β release). However, only prolonged Mitsui-7 exposure induced a significant release of profibrotic mediators (transforming growth factor-β, platelet derived-growth factor, and osteopontin) in all tested cell cultures and concentrations, highlighting the importance of prolonged exposure studies in nanosafety assessment. This elevated release of profibrotic markers was not accompanied by an increased collagen production or cell proliferation in fibroblasts in the tested time frame. Conclusion: Overall, prolonged exposure to human-relevant CNT concentrations appears to be a highly suitable approach to predict profibrotic response in vitro .
机译：简介:微的扩大使用在各种消费和碳纳米管(热合)工业产品引起了担心后的潜在健康风险吸入纳米材料。表明MWCNT暴露可引起肺有害的影响。相关的疾病之一吸入的古典纤维异形材料(如石棉),最近热合。和方法:在此,提出了工作研究肝纤维化的早期迹象在关键人肺细胞类型涉及直接(成纤维细胞)或间接(肺泡上皮细胞和巨噬细胞)在肺癌的发病机制纤维化,急性(24小时)和长期(96小时)暴露在不同浓度(5 - 20μg / mL)的刚性Mitsui-7热合。造成的结果进行了比较相同的细胞接触后混乱的纳米纤维,nanocyl - 7000,和一个积极的控制,结晶石英颗粒(例如,Min-U-Sil)。细胞类型测试,尤其是单身碳纳米管被每个细胞容易内化类型。巨噬细胞炎性反应在24小时(β版本)。长时间接触Mitsui-7诱导显著释放profibrotic介质(转换生长因子β,血小板derived-growth因素,和骨桥蛋白)在所有细胞培养和测试的浓度,突出的重要性长期暴露在nanosafety学习评估。标记并不是伴随着增加胶原蛋白生产或细胞增殖成纤维细胞的检测时间。总的来说,长期接触human-relevant问浓度似乎是一个高度合适方法体外预测profibrotic响应。

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