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首页> 外文期刊>Applied in vitro toxicology. >Pharmacokinetic Evaluation of Ofloxacin in an Invertebrate Animal Model Silkworm, Bombyx mori
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Pharmacokinetic Evaluation of Ofloxacin in an Invertebrate Animal Model Silkworm, Bombyx mori

机译：氧氟沙星的药代动力学评价无脊椎动物模型蚕,家蚕

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We propose silkworm as an animal model for screening the drug molecules, especially antibiotics, because they exhibit a conserved immune response and similar pharmacokinetics (PK) as mammals and importantly no ethical issues are associated. To evaluate the PK parameters and effectiveness of ofloxacin in silkworm. Silkworms with their ideal body weight of 3.8 g were injected with 40 μL of 2 mg/mL ofloxacin. The hemolymph was collected at different time intervals 0 to 300 minutes. The samples were used for estimating the retention of ofloxacin in silkworm hemolymph by high performance liquid chromatography (HPLC), ultra violet visible (UV-VIS) spectrophotometry, liquid chromatography mass spectroscopy (LC-MS), and Fourier transform infrared spectroscopy (FTIR). PK analysis was performed by using the PK solver MS-Excel add-on software. The HPLC method was standardized for the quantification of ofloxacin in silkworm. Further with reference, the structure of ofloxacin was elucidated on the basis of LC-MS spectral data. We reconfirmed the presence and activity of the ofloxacin in silkworm by UV-VIS spectrophotometry and FTIR spectroscopy. The noncompartmental analysis technique was used to quantitatively evaluate and predict the in vivo fate of drug by modeling the concentration time–data with suitable model. The features of the silkworm described suggest that they are a suitable alternative evaluation model for determining the therapeutic effectiveness of candidate drugs. The availability of a large number of silkworms at low cost and no associated ethical issues makes it a better animal model suitable to screen novel antibiotics for their therapeutic effectiveness.
机译：我们建议蚕作为动物模型筛选药物分子,特别是抗生素,因为他们表现出一种守恒的免疫反应和类似的药物动力学(PK)作为哺乳动物和重要的是没有道德问题相关联的。氧氟沙星在蚕的有效性。与他们的理想体重3.8克注射40μL 2毫克/毫升氧氟沙星。血淋巴收集在不同的时间间隔0到300分钟。估算氧氟沙星的保留通过高效液相蚕血淋巴色谱法(高效液相色谱),紫外线可见(紫外分光光度法,液相色谱法质谱分析(质),傅里叶变换红外光谱(红外光谱)。通过使用PK的能手ms excel插件执行的软件氧氟沙星在蚕的量化。与参考,进一步的结构氧氟沙星是阐明的基础上质光谱数据。活动通过紫外可见氧氟沙星的蚕分光光度法和红外光谱。noncompartmental分析技术被用来定量评估和预测体内由建模浓度药物的命运时间数据与合适的模型。表明他们是一个描述的蚕合适的替代评价模型确定治疗的有效性候选药物。蚕在低成本和不相关伦理问题使得它更好的动物模型适合他们的筛选新的抗生素治疗的有效性。

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