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Air–Liquid Interface In Vitro Models for Respiratory Toxicology Research: Consensus Workshop and Recommendations

机译:气液界面的体外模型呼吸系统毒理学研究:共识车间和建议

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摘要

In vitro air–liquid interface (ALI) cell culture models can potentially be used to assess inhalation toxicology endpoints and are usually considered, in terms of relevancy, between classic (i.e., submerged) in vitro models and animal-based models. In some situations that need to be clearly defined, ALI methods may represent a complement or an alternative option to in vivo experimentations or classic in vitro methods. However, it is clear that many different approaches exist and that only very limited validation studies have been carried out to date. This means comparison of data from different methods is difficult and available methods are currently not suitable for use in regulatory assessments. This is despite inhalation toxicology being a priority area for many governmental organizations. In this setting, a 1-day workshop on ALI in vitro models for respiratory toxicology research was organized in Paris in March 2016 to assess the situation and to discuss what might be possible in terms of validation studies. The workshop was attended by major parties in Europe and brought together more than 60 representatives from various academic, commercial, and regulatory organizations. Following plenary, oral, and poster presentations, an expert panel was convened to lead a discussion on possible approaches to validation studies for ALI inhalation models. A series of recommendations were made and the outcomes of the workshop are reported.
机译:体外气液界面(ALI)细胞培养模型可以用来评估吸入毒理学端点和通常认为,在相关性方面,两者之间经典的(例如,淹没)和体外模型动物模型。明确定义,阿里方法可能代表体内的补充或替代选项体外实验或经典方法。然而,很明显,许多不同方法,只有非常有限的存在验证进行了研究。这意味着从不同的比较数据方法是困难的和可用的方法目前在监管不适合使用评估。毒理学是一个优先领域很多政府组织。研讨会以阿里的体外模型呼吸系统毒理学研究组织巴黎在2016年3月对情况进行评估讨论可能的验证研究。主要政党在欧洲和在一起超过60代表各种学术,商业和监管机构。后全体、口头和海报报告,召开了一个专家小组来主要讨论可能的方法阿里吸入模型验证研究。的一系列建议研讨会的结果报道。

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