...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biology of Reproduction: Offical Journal of the Society for the Study of Reproduction >Impact of dietary fatty acids on oocyte quality and development in lactating dairy cows.
【24h】

Impact of dietary fatty acids on oocyte quality and development in lactating dairy cows.

机译：膳食脂肪酸对卵母细胞质量的影响和发展在泌乳奶牛。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The purpose of this study was to examine the effects of level of rumen inert fatty acids on developmental competence of oocytes in lactating dairy cows. Estrous cycles were synchronized in 22 cows on a silage-based diet supplemented with either low (200 g/day) or high (800 g/day) fat. A total of 1051 oocytes were collected by ultrasound-guided ovum pickup (OPU) in seven sessions/cow at 3-4 day intervals. Oocytes were matured, fertilized, and cultured to the blastocyst stage in vitro. Embryo quality was assessed by differential staining of Day 8 blastocysts. The high-fat diet reduced numbers of small and medium follicles. There was no effect on the quality of oocytes (grades 1-4) or cleavage rate. However, high fat significantly improved blastocyst production from matured (P < 0.005) and cleaved (P < 0.05) oocytes. Blastocysts from the high-fat group had significantly more total, inner cell mass and trophectoderm cells than the low-fat group (P < 0.05). Regression analysis showed negative effects of milk yield (P < 0.001), dry matter intake (P < 0.001), metabolizable energy intake (P < 0.005), and starch intake (P < 0.001) on blastocyst production in the low-fat group but not in the high-fat group. Within the low-fat group, blastocyst production was negatively related to growth hormone (P < 0.05) and positively related to leptin (P < 0.05). The low-fat group had higher nonesterified fatty acids than the high-fat group (P < 0.05). In conclusion, higher milk yields were associated with reduced developmental potential of oocytes in cows given a low-fat diet. Provision of a high-fat diet buffered oocytes against these effects, resulting in significantly improved developmental potential.
机译：本研究的目的是检查瘤胃惰性脂肪酸水平的影响在哺乳期卵母细胞的发育能力奶牛。22日牛silage-based饮食补充低(200克/天)或高脂肪(800克/天)。收集的1051个卵母细胞超声引导下卵皮卡(含油率)7会议/牛每隔3 - 4天。成熟、受精和培养的体外胚泡阶段。评估差染色的第八天胚泡。中小型毛囊。在卵母细胞(等级1 - 4)或质量卵裂率。改善从成熟胚泡生产(P <0.005)和裂解卵母细胞(P < 0.05)。从高脂肪组胚泡更,内细胞团滋养外胚层细胞比低脂组(P <0.05)。牛奶产量的影响(P < 0.001),干物质摄入(P < 0.001),代谢能量摄入(P < 0.005),淀粉摄入量(P < 0.001)生产在低脂组胚泡不是高脂肪组。组胚泡生产是消极的生长激素相关(P < 0.05)瘦素正相关(P < 0.05)。低脂组有更高的nonesterified脂肪酸比高脂组(P < 0.05)。结论,提高牛奶产量有关降低卵母细胞的发育潜力在奶牛给予低脂饮食。高脂肪饮食对这些缓冲卵母细胞效果,从而显著提高发展潜力。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号