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首页> 外文期刊>The journal of applied laboratory medicine. >Inactivation of Blood-Borne Enveloped Viruses with the Nonionic Detergent 2-4-(2,4,4-Trimethylpentan-2-yl)PhenoxyEthanol Does Not Bias Clinical Chemistry Results
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Inactivation of Blood-Borne Enveloped Viruses with the Nonionic Detergent 2-4-(2,4,4-Trimethylpentan-2-yl)PhenoxyEthanol Does Not Bias Clinical Chemistry Results

机译：的血源性包膜病毒失活非离子的洗涤剂2 - 4 - (2,4,4-Trimethylpentan-2-yl) Phenoxy乙醇不偏向临床化学结果

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Background: Patients infected with virulent pathogens require the sophisticated diagnostic capabilities of a core laboratory for optimal care. This is especially true in outbreaks that strain healthcare system capacity. However, samples from such patients pose an infection risk for laboratory workers. We evaluated a strategy for mitigating this risk by preincubating specimens with 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol, a non-ionic detergent commonly calledTriton X-100. Methods: Lithium-heparinized plasma was mixed with the detergent Triton X-100 at 1%. Inactivation of Ebola virus (EBOV), yellow fever virus (YFV), and chikungunya virus (CHIKV) was assessed using a virus-outgrowth assay. The impact of 1% Triton X-100 dilution on the components of a complete metabolic panel (CMP) was assessed on a Roche Cobas analyzer with 15 specimens that spanned a large portion of the analytical measurement range. Results: Incubation with 1% Triton X-100 for 5 min was sufficient to completely inactivate EBOV and YFV spiked into plasma but did not completely inactivate CHIKV infectivity even after 60 min of incubation. This was true only for CHIKV when spiked into plasma; CHIKV was completely inactivated in cell culture medium. A bias of -0.78 mmol/L (95% CI, -2.41 to 0.85) was observed for CO2 and 5.79 U/L (95% CI, -0.05 to 11.63) was observed for aspartate aminotransferase after addition of Triton X-100. No other components of the CMP were affected by the addition of Triton X-100. Conclusions: Detergent-based inactivation of plasma specimens may be a viable approach to mitigating the risk that certain blood-borne pathogens pose to laboratory workers in an outbreak setting. However, the effectiveness of this method for inactivation may depend on the specimen type and pathogen in question.
机译：背景:病人感染的毒性病原体需要复杂的诊断最优的核心实验室的功能护理。医疗系统容量。这类病人的样本构成感染的风险实验室工作人员。供preincubating减轻这种风险标本和2 - [4 - (2, 4, 4-trimethylpentan-2-yl)苯氧基]乙醇,一般非离子去污剂calledTritonx - 100。混合洗涤剂Triton x - 100为1%。埃博拉病毒失活(EBOV),黄热病病毒(YFV),基孔肯雅病毒(CHIKV)使用virus-outgrowth试验评估。特里同x - 100稀释1%的影响组件的一个完整的代谢小组(CMP)评估与15罗氏Cobas分析仪吗标本,跨越了很大一部分分析测量范围。有1% Triton x - 100 5分钟足以完全灭活EBOV YFV飙升等离子体,但没有完全灭活CHIKV传染性即使60分钟的孵化。是真的只有CHIKV当上升到等离子体;在细胞培养CHIKV完全灭活媒介。0.85)观察二氧化碳和5.79 U / L(95%可信区间,-0.05到11.63)观察天冬氨酸转氨酶后添加Triton x - 100。没有影响CMP的其他组件添加Triton x - 100。Detergent-based失活的血浆标本可能是一个可行的方法来减轻风险某些血源性病原体构成实验室工作人员在爆发。然而,这种方法的有效性失活可能取决于标本类型和病原体。

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