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首页> 外文期刊>Biology of Reproduction: Offical Journal of the Society for the Study of Reproduction >Profiling gene expression during the differentiation and development of the murine embryonic gonad
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Profiling gene expression during the differentiation and development of the murine embryonic gonad

机译：分析基因表达中小鼠的分化和发展胚胎性腺

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摘要

The application of microarray technology to the study of mammalian organogenesis can provide greater insights into the steps necessary to elicit a functionally competent tissue. To this end, a temporal profile of gene expression was generated with the purpose of identifying changes in gene expression occurring within the developing male and female embryonic gonad. Gonad tissue was collected from mouse embryos at 11.5, 12.5, 14.5, 16.5, and 18.5 days postcoitum (dpc) and relative steady-state levels of mRNA were determined using the Affymetrix MGU74v2 microarray platform. Statistical analysis produced 3693 transcripts exhibiting differential expression during male and/or female gonad development. At 11.5 dpc, the gonad is morphologically indifferent, but at 12.5 dpc, transitions to a male or female phenotype are discernible by the appearance of testicular cords. A number of genes are expressed during this period and many share similar expression profiles in both sexes. As expected, the expression of two well-known sex determination genes, specifically Sry and Sox9, is unique to the testis. Beyond 12.5 dpc, differential gene expression becomes increasingly evident as the male and female tissue morphologically and physiologically diverges. This is evident by two unique waves of transcriptional activity occurring after 14.5 dpc in the male and female. With this study, a large number of transcripts comprising the murine transcriptome can be examined throughout male and female embryonic gonad development and allow for a more complete description of gonad differentiation and development.
机译：微阵列技术的应用研究哺乳动物器官形成可以提供更大的洞察所需的步骤引出一个功能有能力组织。最终,基因表达的时间剖面生成与识别变化的目的在基因表达中发生发展中男性和女性胚胎性腺。组织收集从小鼠胚胎为11.5,12.5, 14.5, 16.5, 18.5天postcoitum (dpc)和相对稳态的mRNA水平决定使用Affymetrix MGU74v2微阵列平台。生产了3693成绩单表现出差异在男性和女性的性腺表达发展。形态学漠不关心,但在12.5 dpc,转换到一个男性或女性表型出现明显的睾丸绳子。这一时期,许多相似的表情配置文件在两性。表达式的两个著名的性别决定基因,特别是Sry和Sox9,是独一无二的睾丸。的表情变得越来越明显男性和女性的组织形态生理上是发散的。独特的转录活动发生在14.5 dpc的男性和女性。与这项研究中,大量的记录组成鼠转录组研究了在男性和女性胚胎性腺发育和允许一个更完整性腺分化和描述发展。

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