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首页> 外文期刊>Biology of Reproduction: Offical Journal of the Society for the Study of Reproduction >NF-kappa B is activated in the rat testis following exposure to mono-(2-ethylhexyl) phthalate
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NF-kappa B is activated in the rat testis following exposure to mono-(2-ethylhexyl) phthalate

机译：在大鼠睾丸nf -κB被激活后暴露在mono - (2-ethylhexyl)邻苯二甲酸酯

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摘要

The process of spermatogenesis requires a delicate balance of proapoptotic and antiapoptotic signaling to maintain optimal sperm output. A major transcription factor known to regulate numerous apoptosis-related genes is NF-kappaB. Here we show that mono-(2-ethylhexyl) phthalate (MEHP, 1 g/kg) induces translocation of NF-kappaB subunits (p65, p50, and c-Rel) to germ cell nuclei in young rats (Postnatal Day 28) as early as 1 h after exposure. Immunohistochemistry of rat testes exposed to MEHP showed increased p50 and c-Rel presence in spermatocytes and spermatogonia. In addition, there was increased p65 nuclear positivity in Sertoli cells and germ cells after MEHP, while Rel-B localization was unchanged. These alterations correlated with increased nuclear NF-kappaB-binding activity after MEHP exposure, as shown by electrophoretic mobility shift assays of whole-testis nuclear protein extracts. The increased activity of this transcription factor was associated with a transient protection of the seminiferous epithelium manifested as a decreased number of germ cell apoptotic nuclei measured by TUNEL assay 6 h after MEHP exposure. These results suggest that NF-kappaB is involved in the testicular response to MEHP-induced injury.
机译：精子形成的过程需要一个微妙的proapoptotic平衡和凋亡输出信号保持最佳的精子。主要的转录因子调节众多apoptosis-related NF-kappaB基因。在这里,我们表明,mono -邻苯二甲酸二(2-ethylhexyl)(MEHP, 1克/公斤)诱发NF-kappaB易位子单元(p65 p50和:)生殖细胞原子核在年轻的老鼠(产后28天)早1 h后曝光。大鼠睾丸暴露MEHP显示p50增加和:在精母细胞的存在spermatogonia。p65核积极支持细胞和细菌细胞后MEHP, Rel-B本地化不变。核NF-kappaB-binding活动增加由电泳MEHP曝光后,如图所示化验whole-testis核流动转变蛋白质的提取。转录因子有关瞬态保护精上皮细胞表现为数量的下降生殖细胞凋亡细胞核通过TUNEL测量试验后6 h MEHP曝光。表明NF-kappaB参与睾丸应对MEHP-induced受伤。

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