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首页> 外文期刊>Biology of Reproduction: Offical Journal of the Society for the Study of Reproduction >Affinity-dependent alterations of mouse B cell development by noninherited maternal antigen
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Affinity-dependent alterations of mouse B cell development by noninherited maternal antigen

机译：Affinity-dependent改变小鼠的B细胞发展noninherited孕产妇抗原

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摘要

We have examined the passage of maternal cells into the fetus during the gestation and postpartum in mice. Using enhanced green fluorescent protein (EGFP)-transgenic females, we showed that maternal cells frequently gain access to the fetus, mostly in syngeneic pregnancies, but also in allogeneic and out-bred crosses. EGFP-transgenic cells, including B, T, and natural killer cells, can persist until adulthood, primarily in bone marrow and thymus. We then asked whether maternal cells, bearing antigens not inherited by the fetus, influence the development of fetal and neonatal B lymphocytes. We have used the B cell receptor 3-83 mu/delta transgenic mouse model, whose B cells recognize the major histocompatibility complex class I molecules H-2K(k) and H-2K(b). with a high or moderate affinity, respectively. The fate of transgenic B cells in animals exposed to noninherited H-2K(k) or H-2K(b) maternal antigens (NIMA) during gestation and lactation was compared with those of nonexposed controls. in H-2K(k)-exposed fetuses, NIMA-specific transgenic B cells are partially deleted during late gestation. Nondeleted cells have down-modulated their R cell receptor. In contrast, in NIMA H-2K(b) exposed neonates, transgenic B cells present an activated phenotype, including proliferation, upregulation of surface CD69, and preferential localization in the T cell zone of splenic follicles. This state of activation is still clearly detectable up to 3 wk of age. Thus, we show that fetal and neonatal B cell development is affected by maternal cells bearing antigens noninherited by the fetus and that this phenomenon is highly dependent on the affinity of the B cell receptor for the NIMA.
机译：我们已经检查了孕产妇细胞的通道胎儿在妊娠和产后小鼠。荧光蛋白(EGFP)转基因雌性,我们表明,母体细胞经常访问胎儿,主要在同源的怀孕,而且在同种异体和out-bred十字架。EGFP-transgenic细胞,包括T, B自然杀伤细胞,可以持续至成年后,主要在骨髓和胸腺。然后我们问母血细胞,轴承抗原不继承了胎儿,影响胎儿和新生儿B的发展淋巴细胞。3 - 83亩/δ转基因小鼠模型的B细胞识别主要组织相容性我复杂的类分子H-2K (k)和H-2K (b)。分别或中度高的亲和力。转基因动物的B细胞的命运对noninherited H-2K (k)或H-2K (b)孕产妇妊娠和哺乳期间抗原(尼玛)是相比nonexposed控制。在H-2K (k)暴露胎儿,NIMA-specific转基因B细胞中部分删除妊娠后期。down-modulated R细胞受体。相反,在尼玛H-2K (b)接触新生儿,转基因B细胞呈现一个激活表型,包括扩散,upregulation表面CD69,优惠本地化脾毛囊的T细胞区。激活显然仍可检测到3工作的年龄。通过母体细胞B细胞发育就会受到影响轴承由胎儿和抗原noninherited这种现象是高度依赖尼玛的B细胞受体的亲和力。

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