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首页> 外文期刊>Biology of Reproduction: Offical Journal of the Society for the Study of Reproduction >Dynamics of carbohydrate affinities at the cell surface of capacitating bovine sperm cells
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Dynamics of carbohydrate affinities at the cell surface of capacitating bovine sperm cells

机译：动态碳水化合物亲和力的细胞使能够牛精子细胞的表面

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摘要

In vivo capacitation of eutherian sperm cells coincides with changes in carbohydrate-dependent interaction with the oviduct epithelia (fucose-dependent for bovine). Heparin-like glycosaminoglycans (GAG) secreted by the oviduct compete for sperm-oviduct binding and are believed to release capacitated sperm cells from oviduct epithelia. A biochemical assay to quantify the specificity and dynamics of carbohydrate-mediated bovine sperm-oviduct binding is developed. Sperm apical plasma membranes (SPM) were purified by a factor eight and biotinylated carbohydrate probes were used for quantitative evaluation of carbohydrate binding. SPM of fresh sperm showed >12 times higher binding capacity for biotinylated fucose than for LewisA. SPM from fresh sperm also efficiently bound biotinylated fucoidan and mannan. Binding of biotinylated fucose could be inhibited by various mono- and oligosaccharides such as fucoidan, mannan, heparin, maltose, and, to a lesser extent, glucose (50% binding at 0.2 mM, 2 mM, 0.3 mug/ml, 15 mM, 50 mM, respectively). SPM from sperm cells that were in vitro capacitated for 4 h in bicarbonate-enriched media (either with or without 10mug/ml heparin) showed a 70-85% reduction in fucose binding. This was also achieved by follicular fluid or by GAG, both obtained from dominant follicles. Total follicular fluid was much more potent in competing with fucose for sperm binding than the isolated GAG moieties (50% competition at 0.02 mug/ml, 20 mug/ml based on number of GAG moieties, respectively). These results support the hypothesis that in vivo capacitation of sperm cells is regulated by carbohydrate moieties similar to those regulating sperm-oviduct adhesion.
机译：体内的真兽亚纲动物精子获能的精子细胞恰逢carbohydrate-dependent的变化输卵管上皮细胞的相互作用(牛focuse-dependent)。粘多糖(GAG)输卵管分泌的争夺sperm-oviduct绑定和认为释放生产精子细胞输卵管上皮细胞。量化的特异性和动态carbohydrate-mediated牛sperm-oviduct绑定了。膜(SPM)纯化的八倍和生物素化的碳水化合物探针碳水化合物的定量评价绑定。生物素化的聚焦能力更高的约束力而不是LewisA。有效地结合生物素化的和甘露聚糖。被各种mono -和低聚糖摘要研究、甘露聚糖等,肝素,麦芽糖,,在较小程度上,葡萄糖(50%绑定为0.2毫米,2毫米,0.3杯/毫升,15毫米,50毫米,分别)。体外生产bicarbonate-enriched 4 h媒体(有或没有肝素10杯/毫升)显示减少了70 - 85%的热点绑定。也通过卵泡液或呕吐,从优势卵泡。卵泡液更有力与植物竞争比精子绑定孤立的呕吐(50%半个竞争为0.02杯/毫升,20杯/毫升基于数量的插科打诨根,分别)。体内的精子获能精子的假设细胞是由碳水化合物半个类似于调节sperm-oviduct附着力。

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