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首页> 外文期刊>Biology of Reproduction: Offical Journal of the Society for the Study of Reproduction >Linker histone H1FOO is required for bovine preimplantation development by regulating lineage specification and chromatin structure(dagger)
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Linker histone H1FOO is required for bovine preimplantation development by regulating lineage specification and chromatin structure(dagger)

机译：Linker histone H1FOO is required for bovine preimplantation development by regulating lineage specification and chromatin structure(dagger)

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Linker histone H1 binds to the nucleosome and is implicated in the regulation of the chromatin structure and function. The H1 variant H1FOO is heavily expressed in oocytes and early embryos. However, given the poor homology of H1FOO among mammals, the functional role of H1FOO during preimplantation embryonic development remains largely unknown, especially in domestic animals. Here, we find that H1FOO is not only expressed in oocytes and preimplantation embryos but granulosa cells and spermatids in cattle. We then demonstrate that the interference of H1FOO results in preimplantation embryonic developmental arrest in cattle using either RNA editing or Trim-Away approach. H1FOO depletion leads to a compromised expression of critical lineage-specific genes at the morula stage and affects the establishment of cell polarity. Interestingly, H1FOO depletion causes a significant increase in the expression of genes encoding other linker H1 and core histones. Concurrently, there is an increase of H3K9me3 and H3K27me3, two markers of repressive chromatin and a decrease of H4K16ac, a marker of open chromatin. Importantly, overexpression of bovine H1FOO results in severe embryonic developmental defects. In sum, we propose that H1FOO controls the proper chromatin structure that is crucial for the fidelity of cell polarization and lineage specification during bovine preimplantation development.
机译：链接器组蛋白H1与核小体结合涉及染色质的监管结构和功能。大量表达的卵母细胞和早期胚胎。然而,鉴于穷人H1FOO的同源性哺乳动物,H1FOO的功能作用胚胎植入前的胚胎发育仍很大程度上是未知的,尤其是在国内的动物。在这里,我们发现H1FOO不仅表达卵母细胞和胚胎植入前的胚胎,但颗粒在牛细胞和精子。证明H1FOO的干扰导致胚胎植入前的胚胎发育在牛使用RNA被捕编辑或减少的方法。导致损害的表达至关重要桑椹胚阶段,lineage-specific基因影响细胞极性的建立。有趣的是,H1FOO损耗引起在基因的表达显著增加编码其他链接器H1和组蛋白核心。同时,增加H3K9me3和H3K27me3,两个标记的染色质和高压减少H4K16ac,开放的标志染色质。H1FOO导致严重的胚胎发育缺陷。适当的染色质结构,是至关重要的富达的细胞极化和血统规范在牛胚胎植入前的发展。

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