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首页> 外文期刊>Acta crystallographica. Section D, Structural biology >Determining biomolecular structures near room temperature using X-ray crystallography: concepts, methods and future optimization
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Determining biomolecular structures near room temperature using X-ray crystallography: concepts, methods and future optimization

机译：使用X射线晶体学确定接近室温的生物分子结构：概念，方法和未来优化

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For roughly two decades, cryocrystallography has been the overwhelmingly dominant method for determining high-resolution biomolecular structures. Competition from single-particle cryo-electron microscopy and micro-electron diffraction, increased interest in functionally relevant information that may be missing or corrupted in structures determined at cryogenic temperature, and interest in time-resolved studies of the biomolecular response to chemical and optical stimuli have driven renewed interest in data collection at room temperature and, more generally, at temperatures from the protein-solvent glass transition near 200 K to similar to 350 K. Fischer has recently reviewed practical methods for room-temperature data collection and analysis [Fischer (2021), Q. Rev. Biophys. 54, e1]. Here, the key advantages and physical principles of, and methods for, crystallographic data collection at noncryogenic temperatures and some factors relevant to interpreting the resulting data are discussed. For room-temperature data collection to realize its potential within the structural biology toolkit, streamlined and standardized methods for delivering crystals prepared in the home laboratory to the synchrotron and for automated handling and data collection, similar to those for cryocrystallography, should be implemented.
机译：大约二十年,cryocrystallography是占绝对统治地位的方法确定高分辨率的生物分子结构。低温电子显微镜和微电子衍射,增加功能的兴趣可能会丢失或相关信息损坏在低温结构决定的温度和时间分辨的兴趣研究生物分子的化学反应和光学刺激推动新的兴趣在室温和数据收集,更多一般来说,在温度的200 K附近protein-solvent玻璃化转变类似于350 K。室温数据的实用方法收集和分析(菲舍尔(2021),问:牧师。Biophys。54岁的e1)。物理原理和方法,在noncryogenic晶体数据收集温度和一些相关因素翻译结果数据进行了讨论。室温数据集合实现其潜在的结构生物学中工具包,简化和标准化的方法提供晶体在家里做好了准备实验室同步加速器和自动化处理和数据收集,类似cryocrystallography,应该实现。

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