Crystallographic and X-ray scattering study of RdfS, a recombination directionality factor from an integrative and conjugative element

Verdonk Callum J.; Marshall Andrew C.; Ramsay Joshua P.Bond Charles S.

首页> 外文期刊>Acta crystallographica. Section D, Structural biology >Crystallographic and X-ray scattering study of RdfS, a recombination directionality factor from an integrative and conjugative element

【24h】

Crystallographic and X-ray scattering study of RdfS, a recombination directionality factor from an integrative and conjugative element

机译：RdfS的晶体学和X射线散射研究，RdfS是来自整合和共轭元素的复合方向因子

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The recombination directionality factors from Mesorhizobium spp. (RdfS) are involved in regulating the excision and transfer of integrative and conjugative elements. Here, solution small-angle X-ray scattering, and crystallization and preliminary structure solution of RdfS from Mesorhizobium japonicum R7A are presented. RdfS crystallizes in space group P2(1)2(1)2(1), with evidence of eightfold rotational crystallographic/noncrystallographic symmetry. Initial structure determination by molecular replacement using ab initio models yielded a partial model (three molecules), which was completed after manual inspection revealed unmodelled electron density. The finalized crystal structure of RdfS reveals a head-to-tail polymer forming left-handed superhelices with large solvent channels. Additionally, RdfS has significant disorder in the C-terminal region of the protein, which is supported by the solution scattering data and the crystal structure. The steps taken to finalize structure determination, as well as the scattering and crystallographic characteristics of RdfS, are discussed.

机译：重组方向性因素Mesorhizobium spp。(RdfS)参与调节的切除和转移综合和共轭元素。解决方案小角x射线散射和结晶和初步的结构解决方案的RdfS Mesorhizobium日本血吸虫R7A提出了。P2(1) 2(1) 2(1),与8倍的证据旋转晶体/ noncrystallographic对称。利用从头开始模型分子置换了部分模型(三个分子)完成后手动检查显示未建模电子密度。晶体结构的RdfS揭示了一个头部到尾部聚合物形成左撇子superhelices大的溶剂频道。c端区域的重要障碍的蛋白质,这是支持的解决方案散射数据和晶体结构。步骤完成结构的决心,以及散射和晶体RdfS的特点,进行了讨论。

著录项

来源
《Acta crystallographica. Section D, Structural biology》 |2022年第10期|1210-1220|共11页
作者
Verdonk Callum J.; Marshall Andrew C.; Ramsay Joshua P.Bond Charles S.;
展开▼
作者单位

University of Western Australia,Univ Western Australia;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类
关键词

相似文献

外文文献

1. A study on some elements and their phases in sediments of Changjiang Estuary by X-ray spectrometer and X-ray diffractometer [J] . 海洋学报：英文版 . 1997,第1期
2. Optical 2-benzyl-5-hydroxy-4-oxopentanoic acids against carboxypeptidase A:Synthesis,kinetic evaluation and X-ray crystallographic study [J] . 无中国化学快报：英文版 . 2010,第2期
3. Optical 2-benzyl-5-hydroxy-4-oxopentanoic acids against carboxypeptidase A:Synthesis,Kinetic evaluation and X-ray crystallographic study [J] . Shou Feng Wang, JingYi Jin, Zong Hao Zeng, 中国化学快报（英文版） . 2010,第2期
4. Study on Application of Bi-directional Combination Technology Integrating Residue Hydrotreating with Catalytic Cracking RICP [J] . Niu Chuanfeng, Gao Yongcan, Dai Lishun, 中国炼油与石油化工：英文版 . 2008,第1期
5. Study on Application of Bi-directional Combination Technology Integrating Residue Hydrotreating with Catalytic Cracking RICP [J] . Niu Chuanfeng, Gao Yongcan, Dai Lishun, 中国炼油与石油化工（英文版） . 2008,第1期
6. X-RAY CRYSTALLOGRAPHIC STUDIES OF THE PRODUCTS OF OXIDATIVE ADDITIONS OF IODINE TO CYCLIC TRINUCLEAR GOLD(I) COMPLEXES - DIRECTIONAL EFFECTS FOR AU-I-CENTER-DOT-CENTER-DOT-CENTER-DOT-I-AU INTERACTIONS [J] . Vickery JC., Balch AL. Inorganic Chemistry: A Research Journal that Includes Bioinorganic, Catalytic, Organometallic, Solid-State, and Synthetic Chemistry and Reaction Dynamics . 1997,第26期

机译：X-RAY CRYSTALLOGRAPHIC STUDIES OF THE PRODUCTS OF OXIDATIVE ADDITIONS OF IODINE TO CYCLIC TRINUCLEAR GOLD(I) COMPLEXES - DIRECTIONAL EFFECTS FOR AU-I-CENTER-DOT-CENTER-DOT-CENTER-DOT-I-AU INTERACTIONS
7. Solvation of the bismuth(III) ion by water, dimethyl sulfoxide, N,N '-dimethylpropyleneurea, and N,N-dimethylthioformamide. An EXAFS, large-angle X-ray scattering, and crystallographic structural study [J] . Naslund J., Sandstrom M., Persson I. Inorganic Chemistry: A Research Journal that Includes Bioinorganic, Catalytic, Organometallic, Solid-State, and Synthetic Chemistry and Reaction Dynamics . 2000,第18期

机译：Solvation of the bismuth(III) ion by water, dimethyl sulfoxide, N,N '-dimethylpropyleneurea, and N,N-dimethylthioformamide. An EXAFS, large-angle X-ray scattering, and crystallographic structural study
8. Modeling particle scattering structure factor for branched bio-inspired polymers in solution: A small angle X-ray scattering study [J] . Bonaccorsi L., Calandra P., Proverbio E.Lombardo D. Journal of Quantitative Spectroscopy & Radiative Transfer . 2013,第18期

机译：Modeling particle scattering structure factor for branched bio-inspired polymers in solution: A small angle X-ray scattering study

Crystallographic and X-ray scattering study of RdfS, a recombination directionality factor from an integrative and conjugative element

摘要

著录项

相似文献

相关主题

期刊订阅