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首页> 外文期刊>International journal of biotechnology and bioengineering research >DNA Fingerprinting of the Freshwater Mud Eel, Monopterus cuchia (Hamilton) by Randomly Amplified Polymorphic DNA (RAPD) Marker
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DNA Fingerprinting of the Freshwater Mud Eel, Monopterus cuchia (Hamilton) by Randomly Amplified Polymorphic DNA (RAPD) Marker

机译：淡水泥鳗 Monopterus cuchia （Hamilton） 的 DNA 指纹图谱 随机扩增多态性 DNA 标记

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摘要

Knowledge on genetic structure of a fish species is essential for assessing the genetic status and subsequently implementing programs on conservation of the genetic resources as well as selective improvement. Random amplified polymorphic DNA fingerprinting assay was applied to examine genetic variability in the freshwater mud eel, Monopterus cuchia, a vulnerable species in Bangladesh. Upon testing 40 random decamer primers four primers giving reproducible results were selected and used for generating DNA fingerprints from a total of 51 fish collected from the district of Mymensingh, Bangladesh. A total of 39 loci were scored, 30 of which were found to be polymorphic in nature. The average values of Nei's gene diversity, Shannon's Information Index and band sharing based similarity index were 0.285, 0.423 and 88.33% respectively indicating a substantial level of genetic diversity in the studied samples of the vulnerable species. Due to the scarcity of M. cuchia in Bangladesh only 51 individuals were examined in the study however the PCR-RAPD fingerprinting technique has been found to be suitable for assessing the genetic structure of the freshwater mud eel that could be used as a baseline study for taking any program on conservation and stock improvement of this species.
机译：对鱼类的遗传结构对评估至关重要的基因状态和随后实施项目遗传资源的保护以及有选择性的提高。多态DNA指纹图谱分析应用检查淡水的遗传变异性黄鳝,Monopterus cuchia,脆弱的物种在孟加拉国。引物四个引物可重复的结果选择用于生成DNA指纹的收集51鱼从Mymensingh,孟加拉国。共有39个位点被拿下,30发现是多态的。值Nei的基因多样性,香农的信息索引和基于频带分割相似性指数分别为0.285、0.423和88.33%分别表示一个实质性的水平遗传多样性的研究样本脆弱的物种。孟加拉国cuchia只有51人然而在研究PCR-RAPD进行了检查指纹识别技术已被发现适用于评估的遗传结构淡水可以作为黄鳝基线研究采取任何项目保护和改善股票物种。

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