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首页> 外文期刊>Agrica >ISOLATION OF POTENTIAL BIOSURFACTANT BACTERIA FROM RAILWAY DIESEL ENGINE YARD SOIL, ITS PCR DETECTION AND ANTIFUNGAL ACTIVITY RHIZOCTONIA SOLANI OF POTATO
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ISOLATION OF POTENTIAL BIOSURFACTANT BACTERIA FROM RAILWAY DIESEL ENGINE YARD SOIL, ITS PCR DETECTION AND ANTIFUNGAL ACTIVITY RHIZOCTONIA SOLANI OF POTATO

机译：铁路柴油机堆场土壤中潜在生物表面活性剂细菌的分离及其PCR检测及马铃薯根瘤菌（RHIZOCTONIA SOLANI）的抗真菌活性

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摘要

Environmental factors force us to focus on new sustainable eco-friendly approaches such as microbial antagonists for the momagemont of soil-borne pathogens, therefore soil samples were collected near Shakurbasti diesel engine shed yard. New Delhi andnovel biosurfactant bacteria obtained were screened, isolated and processed. Out of 10 SKNDLS (1-10) bacteria, only SKNDLS-08 isolate was grown on GSP agar, MacConkey agar as well as on Pseudomonas agar plates that was identified as putative Pseudomonasspp. and exhibited positive biosurfactant activity through different screening methods i.e. penetration assay, oil displacement (+1.7cm), hemolytic activity (>2cm) and EI24 (80%), positive blue plate agar plate (>2.5cm), modified drop collapse method. The SKNDLS-08 isolate inhibited 44.91 %±0.10 of Rhizoctonia solani mycelial growth within 72h of post inoculation under vitro test and at 3% cone, of culture filtrate in food poisoning assay. PCR detection with Pseudomonas genus (PA-30, 618bp) as well species-specific (PA-31, 956bp) primers confirms the identity as Pseudomonas aeruginosa. SKNDLS-08 isolate is an interesting bacterium to investigate for the genes involved in secondary metabolite production in other diseases management applications in agriculture research.
机译：环境因素迫使我们关注新可持续的环保等方法微生物的momagemont拮抗剂土传病菌,因此土壤样本收集附近Shakurbasti柴油机院子里。获得筛选、分离和加工。10 SKNDLS(1 - 10)细菌,只有SKNDLS-08隔离是生长在GSP琼脂,MacConkey琼脂在假单胞菌琼脂板确认为假定的Pseudomonasspp。表现出积极的生物表面活性剂活动通过不同的筛选方法即渗透驱油试验(+ 1.7厘米),溶血性活动(> 2 cm)和EI24(80%)、积极的蓝色板琼脂板(> 2.5厘米),修改后的崩溃方法。% 0.10±辣椒菌丝的生长在72 h后接种在体外测试在3%锥,培养滤液的食物中毒试验。属(PA-30 618 bp)种专一性(956个基点PA-31)引物确认身份铜绿假单胞菌。有趣的细菌的调查基因参与次生代谢物生产在其他疾病管理应用程序农业研究。

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  • 来源
    《Agrica》 |2020年第6期|39-52|共14页
  • 作者

    Touseef Hussain; Abrar Ahmad Khan;

  • 作者单位

    Plant Pathology and Nematology Section,Department of Botany, Aligarh Muslim University, Aligarh-202002, Uttar Pradesh, India;

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  • 原文格式 PDF
  • 正文语种 英语
  • 中图分类 农业科学;
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