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PlantRNA 2.0: an updated database dedicated to tRNAs of photosynthetic eukaryotes

机译:PlantRNA 2.0:专用于光合真核生物 tRNA 的更新数据库

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摘要

PlantRNA () is a comprehensive database of transfer RNA (tRNA) gene sequences retrieved from fully annotated nuclear, plastidial and mitochondrial genomes of photosynthetic organisms. In the first release (PlantRNA 1.0), tRNA genes from 11 organisms were annotated. In this second version, the annotation was implemented to 51 photosynthetic species covering the whole phylogenetic tree of photosynthetic organisms, from the most basal group of Archeplastida, the glaucophyte Cyanophora paradoxa, to various land plants. tRNA genes from lower photosynthetic organisms such as streptophyte algae or lycophytes as well as extremophile photosynthetic species such as Eutrema parvulum were incorporated in the database. As a whole, about 37 000 tRNA genes were accurately annotated. In the frame of the tRNA genes annotation from the genome of the Rhodophyte Chondrus crispus, non-canonical splicing sites in the D- or T-regions of tRNA molecules were identified and experimentally validated. As for PlantRNA 1.0, comprehensive biological information including 5 '- and 3 '-flanking sequences, A and B box sequences, region of transcription initiation and poly(T) transcription termination stretches, tRNA intron sequences and tRNA mitochondrial import are included.
机译:PlantRNA()是一个综合的数据库转移核糖核酸基因序列检索充分注释的核,plastidial和线粒体基因组的光合作用生物。tRNA基因从11个生物带注释的。第二个版本,注释实现51光合物种覆盖整个系统发育树的光合作用生物,从最基础的群paradoxa,各种各样的陆地植物。较低的光合生物等streptophyte藻类或lycophytes以及极端微生物光合物种等Eutrema parvulum纳入数据库。准确的注释。tRNA基因从基因组的注释Rhodophyte陨石球粒管,非规范拼接的D -或T-regions tRNA的网站分子识别和实验验证。生物信息包括5 ' - 3A和B的侧翼序列,序列,地区的转录起始和聚(T)转录终止延伸,tRNA基因内区序列和tRNA线粒体进口包括在内。

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