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首页> 外文期刊>The Plant Journal >BAHD acyltransferase induced by histone deacetylase inhibitor catalyzes 3-O-hydroxycinnamoylquinic acid formation in bamboo cells
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BAHD acyltransferase induced by histone deacetylase inhibitor catalyzes 3-O-hydroxycinnamoylquinic acid formation in bamboo cells

机译：组蛋白脱乙酰酶抑制剂诱导的BAHD酰基转移酶催化竹细胞中3-O-羟基肉桂酰奎宁酸的形成

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摘要

Suspension-cultured cells of a bamboo species (Bambusa multiplex; Bm) produce 3-O-feruloylquinic acid (3-FQA) and 3-O-p-coumaroylquinic acid (3-pCQA) by treatment with the histone deacetylase inhibitor suberoyl bis-hydroxamic acid (SBHA). Acyltransferases catalyzing the formation of 5-O-hydroxycinnamoylquinic acid esters by transesterification from hydroxycinnamoyl-CoAs to the C-5 hydroxy group of quinic acid (hydroxycinnamoyl-CoA:quinate hydroxycinnamoyltransferase, HQT) have been identified in the biosynthesis of chlorogenic acids and monolignols; however, an HQT that catalyzes the acylation of the C-3 hydroxy group of quinic acid has not been identified previously. In the present study, we purified a native HQT from SBHA-treated Bm cells. The purified enzyme preferentially accepted feruloyl-/p-coumaroyl-CoAs as acyl-donors and quinic acid as the acyl-acceptor, and the enzyme specifically formed 3-FQA and 3-pCQA but not 5-O-hydroxycinnamoylquinic acid esters or esters with shikimic acid. A cDNA (BmHQT1) encoding this HQT was isolated. Although BmHQT1 is a phylogenetically unique member of the BAHD acyltransferase superfamily that does not cluster with other HQTs, functional characterization of the recombinant enzyme verified that BmHQT1 catalyzes the regiospecific formation of 3-O-hydroxycinnamoylquinic acid esters. Transcript levels of BmHQT1 markedly increased in Bm cells cultured in the presence of SBHA. Moreover, elevated acetylation levels of histone H3 were observed in the coding region of BmHQT1 in the presence of SBHA, indicating that the induced accumulation of 3-FQA/3-pCQA by SBHA is caused by transcriptional activation of BmHQT1 by the action of SBHA as a histone deacetylase inhibitor. The results demonstrate the utility of HDAC inhibitors for discovery of cryptic secondary metabolites and unknown biosynthetic enzymes.
机译：悬浮细胞的竹种(Bambusa多路复用;3-O-feruloylquinic酸(3-FQA)和3-O-p-coumaroylquinic酸(3-pCQA)治疗组蛋白脱乙酰酶抑制剂suberoylbis-hydroxamic酸(SBHA)。催化的形成5-O-hydroxycinnamoylquinic酸酯,从hydroxycinnamoyl-CoAs酯交换奎尼酸的c - 5羟基组(hydroxycinnamoyl-CoA:奎尼酸hydroxycinnamoyltransferase HQT)中确定绿原的生物合成酸和monolignols;催化的酰化颈- 3羟基组奎尼酸的还没有被确认之前。本机从SBHA-treated HQT细胞。纯化酶优先接受feruloyl——/ p-coumaroyl-CoAs酰基供体奎尼酸acyl-acceptor,和酶特别是3-FQA和3-pCQA但不形成的5-O-hydroxycinnamoylquinic酸酯或酯莽草酸。HQT是孤立的。系统独特的BAHD成员酰基转移酶总科不集群与其他HQTs功能描述重组酶BmHQT1验证催化regiospecific形成3-O-hydroxycinnamoylquinic酸酯。转录水平的BmHQT1明显增加Bm细胞培养在SBHA的存在。此外,组蛋白乙酰化水平的提升H3观察BmHQT1的编码区在SBHA的存在,表明诱导积累3-FQA / 3-pCQA SBHA由BmHQT1的转录激活引起的SBHA作为组蛋白脱乙酰酶的作用抑制剂。HDAC抑制剂的发现神秘二次代谢物和未知的生物合成酶。

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