...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of cellular physiology. >Lnc2300 is a cis‐acting long noncoding RNA of CYP11A1 in ovarian granulosa cells
【24h】

Lnc2300 is a cis‐acting long noncoding RNA of CYP11A1 in ovarian granulosa cells

机译：Lnc2300 是一种在卵巢颗粒细胞中CYP11A1的顺式作用长链非编码 RNA

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract The high level of progesterone and 17β‐estradiol ratio (P4/E2) in follicular fluid has been considered as a biomarker of follicular atresia. CYP11A1, the crucial gene encoding the rate‐limiting enzyme for steroid hormone synthesis, has been reported differently expressed in the ovary during follicular atresia. However, the regulation mechanism of CYP11A1 expression during follicular atresia still remains unclear. Here, we have demonstrated that lnc2300, a novel pig ovary‐specific highly expressed cis‐acting long noncoding RNA (lncRNA) transcribed from chromosome 7, has the ability to induce the expression of CYP11A1 and inhibit the apoptosis of porcine granulosa cells (GCs). Mechanistically, lnc2300, mainly located in the cytoplasm of porcine GCs, sponges and suppresses the expression of miR‐365‐3p through acting as a competing endogenous RNA (ceRNA), which further relieves the inhibitory effects of miR‐365‐3p on the expression of CYP11A1. Besides, CYP11A1 is validated as a direct functional target of miR‐365‐3p in porcine GCs. Functionally, lnc2300 is an antiapoptotic lncRNA that reduces porcine GC apoptosis by inhibiting the proapoptotic function of miR‐365‐3p. In summary, our findings reveal a cis‐acting regulation mechanism of CYP11A1 through lncRNA, and define a novel signaling pathway, lnc2300/miR‐365‐3p/CYP11A1 axis, which is involved in the regulation of GC apoptosis and follicular atresia.
机译：摘要高水平的孕激素17β雌二醇应承担的比例在卵泡液(P4 / E2)已被视为生物标志物的滤泡吗闭锁。速度限制酶对类固醇激素合成,据报道不同表示在卵巢卵泡闭锁。然而,CYP11A1的监管机制表达式在卵泡闭锁仍不清楚。lnc2300,小说猪卵巢非特定的高度表达了独联体还是代理长非编码RNA (lncRNA)转录从染色体7,有能力诱导CYP11A1的表达和抑制猪颗粒细胞的凋亡(GCs)。从力学上看,lnc2300,主要位于细胞质的猪GCs,海绵和抑制miR量365的表达量通过充当3 p进一步竞争内源性RNA(龙头)缓解miR量365 3 p应承担的抑制效应CYP11A1的表达。直接的功能目标进行验证miR量365在猪GCs 3 p。是一种减少凋亡lncRNA猪吗GC proapoptotic通过抑制细胞凋亡米尔量365量3 p的函数。揭示了cis代理监管机制通过lncRNA CYP11A1,定义一个小说信号通路,lnc2300 /米尔365量3 p / CYP11A1轴,这是参与GC的规定细胞凋亡和卵泡闭锁。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号