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Detection of Enterotoxin Genes in Bacillus species Isolated from Cassava Processing Environment in Nigeria

机译：尼日利亚木薯加工环境中分离芽孢杆菌肠毒素基因的检测

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Introduction: The health challenges associated with cassava products as a common staple food for approximately 70 % of Africans and part of Asia pose a looming danger due to Bacillus enterotoxins' presence in the processing environment. Objective: This study investigated the presence of enterotoxigenic genes, namely, Bacillus cereus enterotoxin T (bceT), hemolysin bl (hblC, hblD), and non-haemolytic enterotoxin {nheA, nheB, and nheC) from Bacillus species isolated from soil of cassava processing environment. Methods: Soil samples from 20 cassava processing sites in Ile-Ife and Modakeke, Nigeria, were collected and cultured on nutrient agar at 37°C for 24 hours. Colonies phenotypically identified as Bacillus were identified using Bacillus-specific 16S rRNA-targeted PCR technique. Screened Bacillus spp were assessed for the presence of enterotoxigenic genes using PCR with previously reported primers. Results: A total of 100 Bacillus isolates were selected from this study, with Bacillus macerans (33 %) showing the highest frequency of occurrence among the identified species, however, 74 isolates were molecularly confirmed as Bacillus. Amongst the 74 molecularly confirmed Bacillus isolates, 28 (37.84 %), 35 (47.30 %), and 37 (50 %) had nhe, hbl, and Acer genes, respectively. Investigation showed that 42 (56.76 %) of the Bacillus species encoded at least one of the screened enterotoxin genes. Conclusion: The presence of these 3 sets of enterotoxin genes in Bacillus isolated from cassava processing sites calls for immediate attention as they could be pivotal in the release of toxins in cassava products, causing lethal effects via consumption. This study demonstrates the possibility of foodborne disease outbreaks in Bacillus toxin-laden cassava products processed under unhygienic conditions.
机译：作品简介:相关的卫生挑战木薯产品作为一种常见的主食大约70%的非洲和亚洲的一部分由于芽孢杆菌构成迫在眉睫的危险肠毒素的存在处理环境。产肠毒素的基因的存在,即蜡样芽胞杆菌肠毒素T (bceT),溶血素提单(hblC hblD)和non-haemolytic肠毒素{nheA、nheB nheC)杆菌物种从土壤分离的木薯加工环境。木薯加工网站Ile-Ife Modakeke,尼日利亚、收集和培养的营养琼脂在37°C 24小时。表型鉴定为芽孢杆菌确定使用Bacillus-specific 16 srRNA-targeted PCR技术。spp评估的存在产肠毒素的基因与以前使用PCR报道引物。芽孢杆菌分离株选择从这项研究中,杆菌macerans(33%)最高发生的频率在确认然而,物种分子74隔离确认为芽孢杆菌。确认杆菌分离株,28(37.84%)、35(47.30%)和37例(50%)有新人道,子,宏碁基因,分别。(56.76%)杆菌物种的编码至少有一个的肠毒素基因的筛选。结论:这三组的存在在芽孢杆菌肠毒素基因分离木薯加工网站呼吁立即注意,因为他们可能是关键的毒素在木薯产品,导致致命的通过影响消费。食源性疾病暴发的可能性芽孢杆菌毒素的木薯产品加工在不卫生的条件下。

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