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首页> 外文期刊>Venoms and Toxins >Design, Fabrication and Characterization of Nanoliposomes Containing Snake Venom of Pseudocerastes persicus
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Design, Fabrication and Characterization of Nanoliposomes Containing Snake Venom of Pseudocerastes persicus

机译：含蛇毒的纳米脂质体的设计、制备和表征

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Background: The development of antivenom or antidote requires the repetition of immunization of large animals, such as horses and goats, which ultimately releases the IgG immunoglobulin produced in the serum specimen. As snake venom involves a variety of proteins and enzymes getting administered into the animal, this process can inflict significant harm to the animal; therefore, choosing carriers that can deliver the least amount of venom could be a safer option for animal immunization. Objective: In this research, nanoliposomes were used to encapsulate venom as a protected cargo for immunization. We used two distinct liposomal formulations to entrap the venom: 1,2-dis-tearoyl-sn-glycero-3-phosphocholine, 1,2-distearoyl-sn-glycero-3-phospho-(l '-rac-glycerol) associated with cholesterol in one formulation and dimethyldioctadecylamonium (Bromide salt) paired with cholesterol in the other. Methods: Liposomal formulations were prepared by a solvent evaporation method, and the venom was encapsulated in liposomes and evaluated for size and zeta potential. Meanwhile, encapsulation efficiency, venom release percentage, and phospholipase activity have all been analyzed. Results: The findings revealed that dimethyldioctadecylamonium (Bromide salt) combined with cholesterol had the highest encapsulation efficiency. In this formulation, the venom release rate had a steady-state profile. The lack of phospholipase activity in this formulation may be due to a bromide group in the liposomal structure that could be useful for immunization. Conclusion: Liposomal formulations, which do not have the active site of the snake venom enzymes, could be used for venom encapsulation.
机译：背景:抗蛇毒素或发展解药需要重复接种大型动物,比如马和羊最终释放免疫球蛋白免疫球蛋白产生的血清标本。涉及到各种各样的蛋白质和酶注射到动物,这一点过程可能造成重大伤害动物;最少的毒液可以送货动物免疫安全选项。在这个研究中,nanoliposomes被用来封装毒液为保护货物免疫接种。配方欺骗毒液:1、2-dis-tearoyl-sn-glycero-3-phosphocholine1, 2-distearoyl-sn-glycero-3-phospho - (l“-rac-glycerol)与胆固醇有关一个配方和dimethyldioctadecylamonium(溴化盐)与胆固醇的其他。由溶剂蒸发法,毒液被封装在脂质体和评估对于大小和电动电势。封装效率、释放毒液百分比,磷脂酶活性被分析。dimethyldioctadecylamonium(溴化盐)结合胆固醇最高封装效率。毒液释放率稳态概要文件。这个配方可能由于溴化集团脂质体结构,可能是有用的免疫接种。没有活跃的蛇毒液酶,可用于毒液封装。

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