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首页> 外文期刊>Venoms and Toxins >Top-down and Bottom-up Approaches Revealed New Categories of Pep-tides from the Venom of Moroccan Scorpion Androctonus mauretanicus
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Top-down and Bottom-up Approaches Revealed New Categories of Pep-tides from the Venom of Moroccan Scorpion Androctonus mauretanicus

机译：自上而下和自下而上的方法揭示了来自摩洛哥蝎子Androctonus mauretanicus毒液的新类别

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Background: Androctonus mauretanicus {Am ) is one of the most hazardous scorpions in Morocco and has a highly toxic venom responsible for severe cases of envenomation. However, few studies have focused on deciphering its proteic composition. Objectives: Herein, we aim to map out the complete proteome of the Am venom filtrate to highlight its complexity and the polymorphism of its toxic content. This, in turn, will lead to a deeper understanding of the toxins' mechanism of action and will help uncover those with therapeutic potential. Methods: Top-down and bottom-up proteomic approaches were used complementarily to decipher the proteome of the Am venom. These approaches were carried out on nano-high liquid chromatography coupled to nano-electrospray tandem mass spectrometry (Nano-LC-ESI-MS/MS). Results: Am venom encloses a complex mixture of 269 different compounds with molecular weights ranging from 1618.74 to 14 214.84 Da. The most abundant ones showed masses from 6185.92 to 7899.53 Da (53.89%) followed by those ranging from 2079.25 to 5969.63 Da (37.81%). Interestingly, the combination of the results of both approaches allowed the screening of a total of 112 peptides. The highest percentage was represented by neuropeptides (87%), including NaTxs, KTxs, CITxs, venom proteins, venom neuropeptides, and myotropic neuropeptides. Moreover, other peptides were identified, such as antimicrobial peptides, amphipathic peptides, cysteine-rich venom peptides, enzymes, kunitz-type inhibitors and orphan peptides. Conclusion: The Am venom appears to contain a great number of diverse peptides, some of which could prospectively be exploited for their pharmaceutical potential.
机译：背景:Androctonus mauretanicus{我)就是其中之一摩洛哥和最危险的蝎子有剧毒的毒液负责严重表面变质。专注于破译它的蛋白质的成分。目的:在这里,我们的目标是在地图上标出完整的蛋白质组是毒液滤液突出它的复杂性和多态其不良内容。深入了解毒素的机制行动,将有助于揭示那些治疗的潜力。自底向上的蛋白质组学方法配套破译的蛋白质组毒液。nano-high液相色谱耦合nano-electrospray串联质谱(Nano-LC-ESI-MS / MS)。269种不同的化合物的复杂混合物分子量范围从1618.74 - 14所示214.84哒。从6185.92到7899.53 Da(53.89%)紧随其后这些范围从2079.25到5969.63(37.81%)。这两种方法允许筛选的结果总共112肽。比例是由神经肽蛋白质,毒液神经肽,myotropic神经肽。识别,如抗菌肽,两性分子的肽,cysteine-rich毒液肽酶,kunitz-type抑制剂孤儿肽。包含许多不同的肽,其中一些前瞻性可以利用为他们的药物的潜力。

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