In situ preparation of programmable curli nanomaterials as fine-tuned sustainable supports enabling selective and oriented incorporation of enzymes

Hao Dong; Wenxue Zhang; Chao Chen

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In situ preparation of programmable curli nanomaterials as fine-tuned sustainable supports enabling selective and oriented incorporation of enzymes

机译：In situ preparation of programmable curli nanomaterials as fine-tuned sustainable supports enabling selective and oriented incorporation of enzymes

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Pollution caused by phthalic acid esters (PAEs) has shown interference with hormone homeostasis. To reduce the influence of PAEs on the environment, a two-step method for magnetic curli nanomaterial preparation was developed without excess use of high-salt solutions, which realized the selective and oriented immobilization of esterases. It was achieved by the preparation of Fe3O4@NiSiO3@CsgB nanomaterials, which facilitated the anchoring, assembly, and magnetic recovery of curli nanofibers in situ without complicated post-processing. The resulting magnetic curli nanomaterials achieved the specific immobilization of esterase Est3-14(SpyCatcher) through conjugation pairing (SpyTag/SpyCatcher), with an effective enzyme loading of >329 mg g~(-1). When tested for biodegradation reactions, ～88% of dibutyl phthalate was degraded by immobilized esterases in 1 h. The immobilized enzyme retained over 52% of its activity after eight cycles of reuse. These results demonstrate the feasibility of the structural and property fabrication of green and sustainable curli nanomaterials for biotechnological applications.

机译：污染造成的邻苯二甲酸酯(PAEs)表明干扰激素体内平衡。减少对邻苯二甲酸酯的影响环境中,磁curli两步方法纳米材料的制备是没有开发的过度使用高盐的解决方案,实现的选择性和定向固定化酯酶。Fe3O4@NiSiO3@CsgB纳米材料,促进了锚定、装配和磁性恢复curli原位纳米纤维复杂的后处理。磁curli纳米材料实现了特定的酯酶固定化通过结合配对Est3-14 (SpyCatcher)(SpyTag / SpyCatcher),一个有效的酶加载> 329毫克g ~(1)。生物降解反应,二丁基~ 88%邻苯二甲酸酯被固定化酯酶降解在1 h。固定化酶保留超过52%经过8其活动周期的重用。这些结果证明的可行性绿色制造和结构和属性可持续curli纳米材料生物技术的应用程序。

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《Catalysis science & technology》 |2023年第10期|3139-3146|共8页
作者
Hao Dong; Wenxue Zhang; Chao Chen;
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State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, No. 130 Meilong Road, Xuhui District, Shanghai 200237, People's Republic of China;

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正文语种英语
中图分类物理化学（理论化学）、化学物理学;
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In situ preparation of programmable curli nanomaterials as fine-tuned sustainable supports enabling selective and oriented incorporation of enzymes

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