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>Decreased3H‐uridine incorporation and increased3H‐adenosine incorporation by hela cells exposed to autologous culture fluid
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Decreased3H‐uridine incorporation and increased3H‐adenosine incorporation by hela cells exposed to autologous culture fluid
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机译：Decreased3H‐uridine incorporation and increased3H‐adenosine incorporation by hela cells exposed to autologous culture fluid
AbstractActively growing HeLa monolayer cultures briefly exposed to the culture fluids (CF) from confluent HeLa cultures and labeled simultaneously or subsequently, incorporated less3H‐uridine (3H‐UR) but more3H‐adenosine (3H‐AR) than control cultures similarly exposed to fresh medium and labeled. Exposure to CF inhibited the uptake as well as the incorporation of3H‐UR by cultures. The inhibition of3H‐UR incorporation by CF‐exposed cultures could be reduced by increasing the concentration of3H‐UR in the labeling medium. Both the inhibition of3H‐UR incorporation and the stimulation of3H‐AR incorporation were prevented by washing the CF‐treated cultures with phosphate buffered saline before labeling. Similarly, both effects could be producted in HeLa cultures exposed to fresh medium containing 1 × 10−5M uridine instead of to CF. Therefore, the observed effects of CF on label incorporation were probably due to the presence of uridine or a related compound, and the inhibition of3H‐UR incorporation resulted from reduced uptake of3H‐UR rather than from reduced RNA synthesis by exposed cells. The active agent in the CF, formed only when cultures were incubated at physiological temperatures, was not a product of medium decay. It was a cellular product formed equally well by cultures incubated in medium contai
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