Metabolomics of red‐light‐induced stomatal opening in        i Arabidopsis thaliana/iArabidopsis thaliana        : Coupling with abscisic acid and jasmonic acid metabolism

Zhu Mengmeng; Geng Sisi; Chakravorty DavidGuan QijieChen SixueAssmann Sarah M.

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Metabolomics of red‐light‐induced stomatal opening in i Arabidopsis thaliana/iArabidopsis thaliana : Coupling with abscisic acid and jasmonic acid metabolism

机译：Metabolomics of red‐light‐induced stomatal opening in i Arabidopsis thaliana/iArabidopsis thaliana : Coupling with abscisic acid and jasmonic acid metabolism

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Summary Environmental stimuli‐triggered stomatal movement is a key physiological process that regulates CO 2 uptake and water loss in plants. Stomata are defined by pairs of guard cells that perceive and transduce external signals, leading to cellular volume changes and consequent stomatal aperture change. Within the visible light spectrum, red light induces stomatal opening in intact leaves. However, there has been debate regarding the extent to which red‐light‐induced stomatal opening arises from direct guard cell sensing of red light versus indirect responses as a result of red light influences on mesophyll photosynthesis. Here we identify conditions that result in red‐light‐stimulated stomatal opening in isolated epidermal peels and enlargement of protoplasts, firmly establishing a direct guard cell response to red light. We then employ metabolomics workflows utilizing gas chromatography mass spectrometry and liquid chromatography mass spectrometry for metabolite profiling and identification of Arabidopsis guard cell metabolic signatures in response to red light in the absence of the mesophyll. We quantified 223 metabolites in Arabidopsis guard cells, with 104 found to be red light responsive. These red‐light‐modulated metabolites participate in the tricarboxylic acid cycle, carbon balance, phytohormone biosynthesis and redox homeostasis. We next analyzed selected Arabidopsis mutants, and discovered that stomatal opening response to red light is correlated with a decrease in guard cell abscisic acid content and an increase in jasmonic acid content. The red‐light‐modulated guard cell metabolome reported here provides fundamental information concerning autonomous red light signaling pathways in guard cells.

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《The Plant Journal》 |2020年第6期|1331-1348|共18页
作者
Zhu Mengmeng; Geng Sisi; Chakravorty DavidGuan QijieChen SixueAssmann Sarah M.;
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作者单位

Department of BiologyPennsylvania State UniversityUniversity Park,PA,16802,USA;

The Plant Molecular and Cellular Biology ProgramUniversity of FloridaGainesville,FL,32610,USA;

Department of BiologyUniversity of FloridaGainesville,FL,32610,USA;

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原文格式 PDF
正文语种英语
中图分类植物学;
关键词
abscisic acid; Arabidopsis thaliana; guard cell; jasmonic acid; metabolomics; K + channel; red light; stomata;

Metabolomics of red‐light‐induced stomatal opening in i Arabidopsis thaliana/iArabidopsis thaliana : Coupling with abscisic acid and jasmonic acid metabolism

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