Two loss-of-function alleles of the glutathione S-transferase (GST) gene cause anthocyanin deficiency in flower and fruit skin of peach (Prunus persica)

Zhenhua Lu; Huihui Cao; Lei PanLiang NiuBin WeiGuoChao CuiLuwei WangJia-Long YaoWenfang ZengZhiqiang Wang

首页> 外文期刊>The Plant Journal >Two loss-of-function alleles of the glutathione S-transferase (GST) gene cause anthocyanin deficiency in flower and fruit skin of peach (Prunus persica)

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Two loss-of-function alleles of the glutathione S-transferase (GST) gene cause anthocyanin deficiency in flower and fruit skin of peach (Prunus persica)

机译：Two loss-of-function alleles of the glutathione S-transferase (GST) gene cause anthocyanin deficiency in flower and fruit skin of peach (Prunus persica)

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Flower and fruit colors are important agronomic traits. To date, there is no forward genetic evidence that the glutathione S-transferase (GST) gene is responsible for the white flower color in peach (Prunus persica). In this study, genetic analysis indicated that the white-flower trait is monogenetic, is recessive to the non-white allele, and shows pleiotropic effects with non-white-flowered types. The genetic locus underpinning this trait was mapped onto chromosome 3 between 0.421951 and 3.227115 Mb by using bulked segregant analysis in conjunction with whole-genome sequencing, and was further mapped between 0 and 1.178149 Mb by using the backcross 1 (BC_1) population. Finally, the locus was fine-mapped within 535.974-and 552.027-kb intervals by using 151 F2 individuals and 75 individuals from a BC_1 self-pollinated (BC_1S_1) population, respectively. Pp3G013600, encoding a GST that is known to transport anthocyanin, was identified within the mapping interval. The analysis of genome sequence data showed Pp3G013600 in white flowers has a 2-bp insertion or a 5-bp deletion in the third exon. These variants likely render the GST nonfunctional because of early stop codons that reduce the protein length from 215 amino acids to 167 and 175 amino acids, respectively. Genetic markers based on these variants validated a complete correlation between the GST loss-of-function alleles and white flower in 128 peach accessions. This correlation was further confirmed by silencing of Pp3G013600 using virus-induced gene silencing technology, which reduced anthocyanin accumulation in peach fruit. The new knowledge from this study is useful for designing peach breeding programs to generate cultivars with white flower and fruit skin.

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来源
《The Plant Journal》 |2021年第5期|1320-1331|共12页
作者
Zhenhua Lu; Huihui Cao; Lei PanLiang NiuBin WeiGuoChao CuiLuwei WangJia-Long YaoWenfang ZengZhiqiang Wang;
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作者单位

National Peach and Grape Improvement Center/Key Laboratory of Fruit Breeding Technology of Ministry of Agriculture, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, China;

Tangshan Food and Drug Comprehensive Testing Center, Tangshan, China;

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正文语种英语
中图分类植物学;
关键词
fruit skin coloration; glutathione S-transferase; Bulked Segregant Analysis strategy coupled with whole-genome resequencing; peach; anthocyanin;

Two loss-of-function alleles of the glutathione S-transferase (GST) gene cause anthocyanin deficiency in flower and fruit skin of peach (Prunus persica)

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