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Dual-wavelength digital holographic imaging with phase background subtraction

机译：具有相位背景减法的双波长数字全息成像

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摘要

Three-dimensional digital holographic microscopic phase imaging of objects that are thicker than the wavelength of the imaging light is ambiguous and results in phase wrapping. In recent years, several unwrapping methods that employed two or more wavelengths were introduced. These methods compare the phase information obtained from each of the wavelengths and extend the range of unambiguous height measurements. A straightforward dual-wavelength phase imaging method is presented which allows for a flexible tradeoff between the maximum height of the sample and the amount of noise the method can tolerate. For highly accurate phase measurements, phase unwrapping of objects with heights higher than the beat (synthetic) wavelength (i.e. the product of the original two wavelengths divided by their difference), can be achieved. Consequently, three-dimensional measurements of a wide variety of biological systems and microstructures become technically feasible. Additionally, an effective method of removing phase background curvature based on slowly varying polynomial fitting is proposed. This method allows accurate volume measurements of several small objects with the same image frame.
机译：比成像光波长厚的物体的三维数字全息显微相位成像是模糊的，并导致相位包裹。近年来，引入了几种采用两种或更多波长的解包方法。这些方法比较从每个波长获得的相位信息，并扩展了明确高度测量的范围。提出了一种简单的双波长相位成像方法，该方法允许在样品的最大高度和该方法可以容忍的噪声量之间灵活权衡。对于高精度的相位测量，可以实现高度高于拍频（合成）波长（即原始两个波长的乘积除以它们的差值）的物体的相位展开。因此，对各种生物系统和微观结构进行三维测量在技术上变得可行。此外，提出了一种基于缓慢变化多项式拟合的相位背景曲率去除方法。这种方法可以对具有相同图像帧的几个小物体进行精确的体积测量。

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