首页> 外文期刊>Journal of Rapid Methods and Automation in Microbiology >Evaluation of Beadretriever (TM), an automated system for concentration ofEscherichia coli O157 from enrichment cultures by immunomagneticseparation
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Evaluation of Beadretriever (TM), an automated system for concentration ofEscherichia coli O157 from enrichment cultures by immunomagneticseparation

机译:Evaluation of Beadretriever (TM), an automated system for concentration ofEscherichia coli O157 from enrichment cultures by immunomagneticseparation

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摘要

The aim of this study was to compare automated immunomagnetic separation (AIMS) with manual IMS (MIMS) for detection of E. coli O157 in natural and artificially inoculated samples. E. coli O157 was isolated from 27 (14.7%) of 184 natural bovine fecal samples by MIMS and from 26 (14.1%) by AIMS. Of 94 natural carcass samples, E. coli O157 was isolated from 6 by both MIMS and AIMS and from a further 6 by AIMS only. Three of the six positive only by AIMS were from tubes processed adjacently to one containing an inoculated sample and strains isolated from these adjacent tubes were indistinguishable by various typing techniques. Of the 36 carcass samples inoculated with E. coli O157, the organism was isolated from 25 by both MIMS and AIMS and from a further 9 by AIMS only. To process 100 samples by MIMS took 5 h whereas by AIMS this took only 3 h 20 min which represented a considerable saving of staff time.

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