A Novel Electrochemical Method for the Sensitive Determination of Aflatoxin B1 Using a Bivalent Binding Aptamer‐cDNA Structure

Elham Sameiyan; Parirokh Lavaee; Mohammad RamezaniMona AlibolandiZahra KhoshbinKhalil AbnousSeyed Mohammad Taghdisi

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A Novel Electrochemical Method for the Sensitive Determination of Aflatoxin B1 Using a Bivalent Binding Aptamer‐cDNA Structure

机译：A Novel Electrochemical Method for the Sensitive Determination of Aflatoxin B1 Using a Bivalent Binding Aptamer‐cDNA Structure

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Abstract In this study, a simple electrochemical sensing platform with the employment of a bivalent binding aptamer‐cDNA probe (BBA‐cDNA) structure is constructed for the detection of aflatoxin B1 (AFB1) as a mycotoxin. The BBA‐cDNA structure is composed of two strands of aptamer (Apts) and their complementary strand (CS). Using a simple but accurate design, the presented measurement approach showed enhanced sensitivity and selectivity for AFB1 detection with a LOD of 0.1 ng/mL. The approach presented in this study can be applied to the development of biosensors for the measurement of various toxins by substituting the proper aptamers and complementary strands.

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来源
《Electroanalysis》 |2023年第3期|n/a-n/a|共8页
作者
Elham Sameiyan; Parirokh Lavaee; Mohammad RamezaniMona AlibolandiZahra KhoshbinKhalil AbnousSeyed Mohammad Taghdisi;
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作者单位

Mashhad University of Medical Sciences;

Research Institute for Industrial Biotechnology;

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正文语种英语
中图分类电化学、电解、磁化学;
关键词
Electrochemical aptasensor; Methylene blue; Aflatoxin B1; BBA-cDNA structure;

A Novel Electrochemical Method for the Sensitive Determination of Aflatoxin B1 Using a Bivalent Binding Aptamer‐cDNA Structure

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