Effect of fenofibrate on proliferation of SMMC-7721 cells via regulating cell cycle

B Li; H-Y Jiang; Z-H WangY-C MaY-N BaoY Jin

首页> 外文期刊>Human and Experimental Toxicology >Effect of fenofibrate on proliferation of SMMC-7721 cells via regulating cell cycle

【24h】

Effect of fenofibrate on proliferation of SMMC-7721 cells via regulating cell cycle

机译：Effect of fenofibrate on proliferation of SMMC-7721 cells via regulating cell cycle

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Liver cancer is a malignant cancer with great harmfulness. Fenofibrate is a peroxisome proliferation activated receptor (PPARα) agonist widely used in the treatment of dyslipidemia. Previous studies have shown that fenofibrate may promote cell proliferation, but the underlying mechanism has not been fully characterized. The aim of this study was to investigate the role of PPARα agonist fenofibrate in cell proliferation of SMMC-7721 cells compared with that of THLE-2 cells. SMMC-7721 and THLE-2 cells were treated with different concentrations of fenofibrate. Cell proliferation was analyzed by MTT, using flow cytometry for cell cycle analysis, and CyclinD1, Cyclin-dependent kinases2 (CDK2) and Proliferating Cell Nuclear Antigen (PCNA) were analyzed by Western blotting. RT-qPCR method was used to assess CDK2, CyclinD1 and PCNA mRNA levels. The results showed that 10~(?9)–10~(?4)mol/L fenofibrate could induce cell growth and 10~(?4), 10~(?5), 10~(?6)mol/L fenofibrate could reduce the number of G0/G1 phase cells and increased in the number of cells in S and G2/M phase of cell cycle in SMMC-7721 cells. Furthermore, fenofibrate could significantly increase the expression of cell cycle related protein (CyclinD1, CDK2)and cell proliferation related proteins (PCNA). The use of PPARα inhibitor MT886 inhibited cell cycle progression and promote tumor cell apoptosis. But fenofibrate had no obvious effect on THLE-2 cells. These results revealed the effect of fenofibrate on the cell cycle of liver cancer cells, and provided a reasonable explanation for studying how fenofibrate promotes cell proliferation.

著录项

来源
《Human and Experimental Toxicology》 |2021年第7期|1208-1221|共14页
作者
B Li; H-Y Jiang; Z-H WangY-C MaY-N BaoY Jin;
展开▼
作者单位

Key Laboratory of Anti-inflammatory and Immune Medicines, Ministry of Education, School of Pharmacy;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类毒物学（毒理学）;
关键词
Fenofibrate; SMMC-7721 cells; THLE-2 cells; cell proliferation; cell cycle; tumorigenesis;

Effect of fenofibrate on proliferation of SMMC-7721 cells via regulating cell cycle

摘要

著录项

相关主题

期刊订阅