Recombinant M13Hol phage containingEcoR1 restriction endonuclease fragments B, E, and F of adenovirus type 2 (Ad2) DNA were constructed by cloning into the uniqueEcoR1 site of the replicative form of the phage M13Hol176 DNA. Polarity of the adenovirus inserts in recombinant molecules was deduced by the following procedures: Viral DNA fragments obtained from Ad2 DNA molecules were purified, denatured, and subjected to electrophoresis. the separated DNA strands were transferred from agarose to nitrocellulose by the Southern procedure and hybridized with radioactive 3′‐end labeledHaeIII fragments of the recombinant phage DNAs. This procedure provided a rapid test for assaying strandedness of the cloned fragme
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