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首页> 外文期刊>Archives of Toxicology >Reduction of cisplatin toxicity in cultured renal tubular cells by the bioflavonoid quercetin.
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Reduction of cisplatin toxicity in cultured renal tubular cells by the bioflavonoid quercetin.

机译：生物类黄酮槲皮素可降低培养的肾小管细胞中顺铂的毒性。

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摘要

Quercetin (QC), a polyphenolic compound widely distributed in fruits and vegetables has recently gained interest due to its cisplatin (CP) sensitizing properties in cancer cells. It is currently unknown, whether quercetin also increases the susceptibility of the kidneys to cisplatin toxicity. We studied the effects of various bioflavonoids on CP toxicity in an in vitro model of cultured tubular epithelial cells (LLC-PK1). Viability of LLC-PK1 cells, as assessed by lactate dehydrogenase (LDH) release and MTT-test, was affected by CP (100-400 microM) in a time and dose dependent fashion. Pretreatment of cells with QC for 3 h significantly reduced the extent of cell damage. The protective activity of QC was concentration dependent, starting at 10-25 microM and reaching a plateau between 50 and 100 microM. Other bioflavonoids (catechin, silibinin, rutin) did not diminish cellular injury, even at higher concentrations (100-500 microM). Quercetin itself showed some intrinsic cytotoxicity at concentrations exceeding 75 microM. Our data indicate that quercetin reduces cisplatin toxicity in cultured tubular epithelial cells. The exact mechanism of protection is unclear, though scavenging of free oxygen radicals may play an important role.
机译：槲皮素（QC）是一种广泛分布于水果和蔬菜中的多酚化合物，由于其在癌细胞中的顺铂（CP）敏化特性，最近引起了人们的兴趣。目前尚不清楚槲皮素是否还会增加肾脏对顺铂毒性的敏感性。我们研究了体外培养的肾小管上皮细胞（LLC-PK1）模型中各种生物类黄酮对CP毒性的影响。通过乳酸脱氢酶（LDH）释放和MTT测试评估的LLC-PK1细胞的生存力受CP（100-400 microM）的影响，呈时间和剂量依赖性。用QC预处理细胞3小时可大大减少细胞损伤的程度。 QC的保护活性是浓度依赖性的，起始浓度为10-25 microM，达到50-100 microM的平稳期。其他生物类黄酮（儿茶素，水飞蓟宾，芦丁）即使在较高浓度（100-500 microM）下也不能减轻细胞损伤。槲皮素本身在浓度超过75 microM时显示出某些固有的细胞毒性。我们的数据表明槲皮素可降低培养的管状上皮细胞中的顺铂毒性。尽管清除游离氧自由基可能起重要作用，但确切的保护机制尚不清楚。

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