Anion inhibition study of the β-carbonic anhydrase (CahB1) from the cyanobacterium Coleofasciculus chthonoplastes (ex-Microcoleus chthonoplastes)

VulloD.; KupriyanovaE.V.; ScozzafavaA.CapassoC.SupuranC.T.

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Anion inhibition study of the β-carbonic anhydrase (CahB1) from the cyanobacterium Coleofasciculus chthonoplastes (ex-Microcoleus chthonoplastes)

机译：Anion inhibition study of the β-carbonic anhydrase (CahB1) from the cyanobacterium Coleofasciculus chthonoplastes (ex-Microcoleus chthonoplastes)

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We investigated the catalytic activity and inhibition of the β-class carbonic anhydrase (CA, EC 4.2.1.1) CahB1, from the relict cyanobacterium Coleofasciculus chthonoplastes (previously denominated Microcoleus chthonoplastes). The enzyme showed good activity as a catalyst for the CO 2 hydration, with a kcat of 2.4 × 105 s-1 and a kcat/Km of 6.3 × 107 M-1 s-1. A range of inorganic anions and small molecules were investigated as inhibitors of CahB1. Perchlorate and tetrafluoroborate did not inhibit the enzyme (KIs 200 mM) whereas selenate and selenocyanide were ineffective inhibitors too, with KIs of 29.9-48.61 mM. The halides, pseudohalides, carbonate, bicarbonate, trithiocarbonate and a range of heavy metal ions-containing anions were submillimolar-millimolar inhibitors (KIs in the range of 0.15-0.90 mM). The best CahB1 inhibitors were N,N-diethyldithiocarbamate, sulfamate, sulfamide, phenylboronic acid and phenylarsonic acid, with KIs in the range of 8-75 μM, whereas acetazolamide inhibited the enzyme with a KI of 76 nM. This is the first kinetic and inhibition study of a cyanobacterial CA. As these enzymes are widespread in many cyanobacteria, being crucial for the carbon concentrating mechanism which assures substrate to RubisCO for the CO 2 fixation by these organisms, a detailed kinetic/inhibition study may be essential for a better understanding of this superfamily of metalloenzymes and for potential biotechnological applications in biomimetic CO2 capture processes.

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《Bioorganic and medicinal chemistry》 |2014年第5期|1667-1671|共5页
作者
VulloD.; KupriyanovaE.V.; ScozzafavaA.CapassoC.SupuranC.T.;
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作者单位

Università Degli Studi di Firenze, Dipartmento di Chimica Ugo Schiff, Via della Lastruccia 3, 50019;

K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Str.,;

Istituto di Biochimica Delle Proteine, CNR, Via P. Castellino 111, 80131 Napoli, Italy;

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正文语种英语
中图分类药学;
关键词
β-Class; Anion; Carbon concentrating mechanism; Carbonic anhydrase; CO2 capture; Cyanobacterium; Enzyme inhibitor;

Anion inhibition study of the β-carbonic anhydrase (CahB1) from the cyanobacterium Coleofasciculus chthonoplastes (ex-Microcoleus chthonoplastes)

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