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首页> 外文期刊>Bulletin of Animal Health and Production in Africa >An evaluation of an IgG ELISA for the diagnosis of bovine leptospirosis
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An evaluation of an IgG ELISA for the diagnosis of bovine leptospirosis

机译:An evaluation of an IgG ELISA for the diagnosis of bovine leptospirosis

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摘要

Five hundred and ninety seven abattoir cattle were tested for evidence of leptospiral antibodies using the Microscopic Agglutination Test (MAT) and the Enzyme-Linked Immunosorbent Assay (ELISA) test. One hundred and forty (23.45 percent) of the sampled animals were positive by ELISA technique while 107 (17.92 percent) were reactive in the MAT. However, there was no significant difference (p>0.01, #chi#~2 = 5.56) in the number of reactors by both tests. The sensitivity of the ELISA was found to be 95percent while the specificity was 93.27 percent. Using the MAT, twenty five (23.37 percent) of the seropositive animals reacted with the Leptospira hardjo antigen, while 20 (18.69 percent) and 18 (16.82 percent) were seropositive to L. pomona and L. icterohaemorrhagiae respectively.

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