The adherence of lipopolysaccharides (LPSs) from periodontal disease‐associated bacteria to saliva‐coated hydroxyapatite (S‐HA) and serum‐coated HA beads was examined by chromogenicLimulusactivity (toxicolor test). Phenol‐water extracted LPS preparations fromBacteroides intermedius, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans, Eikenella corrodens, and rough‐type LPS fromEscherichia coliadhered to S‐HA and serum‐coated beads and agglutinated human erythrocytes. The adhered LPSs to S‐HA and serum‐coated HA beads were not removed by vigorous washing with distilled water. LPSs fromBacteroides (Porphyromonas) gingivalisstrains and wild‐typeE. colidid not adhere to S‐HA, serum‐coated HA beads or show hemagglutinating activity. SDS‐PAGE patterns stained with silver stain showed that LPSs adhered to S‐HA, and serum‐coated HA beads and erythrocytes possessed a distinct fast‐migrating band similar to rough‐type LPS.B. gingivalisLPSs possessed slow‐migrating and repeating
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