Rapid Fibroblast Cell Culture Characterization with Impedimetric Label-free Heat Shock Protein-47 Biosensor

Harmanci Duygu; Uygun Zihni Onur; Sezgin Ayse KocakDemirdover CenkSagin Ferhan GirginAkdogan Gul

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Rapid Fibroblast Cell Culture Characterization with Impedimetric Label-free Heat Shock Protein-47 Biosensor

机译：Rapid Fibroblast Cell Culture Characterization with Impedimetric Label-free Heat Shock Protein-47 Biosensor

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In this study, for the first time in literature, an impedimetric biosensor technology was developed to determine Heat Shock Protein-47 (HSP-47) for the characterization of primary skin fibroblast cells. Gold nanoparticle electrodes (GNPE) were modified with amino-functionalized graphene oxide layers and anti-HSP-47 were immobilized on this layer. The performance of the biosensor was tested with real samples as cell lysates. The low detection limit (LOD) and the linear detection limit (LOQ) of the biosensor were calculated as 9.47 pg/mL and 3.23 pg/mL, respectively. Linear measurement range was obtained between 10-160 pg/mL HSP-47. The reproducibility of the biosensor was good.

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来源
《Electroanalysis》 |2020年第10期|2310-2315|共6页
作者
Harmanci Duygu; Uygun Zihni Onur; Sezgin Ayse KocakDemirdover CenkSagin Ferhan GirginAkdogan Gul;
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作者单位

Dokuz Eylul Univ, Grad Sch Hlth Sci, Mol Med Dept, Izmir, Turkey;

Ege Univ, Fac Med, Med Biochem Dept, Izmir, Turkey;

Dokuz Eylul Univ, Sch Med, Plast Reconstruct & Aesthet Surg Dept, Izmir, TurkeyIzmir Univ Econ, Sch Med, Med Biochem Dept, Izmir, Turkey;

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正文语种英语
中图分类电化学、电解、磁化学;
关键词
HSP-47; impedance; gold nanoparticle; graphene; chronoimpedance;

Rapid Fibroblast Cell Culture Characterization with Impedimetric Label-free Heat Shock Protein-47 Biosensor

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