Structural evidences for a secondary gold binding site in the hydrophobic box of lysozyme

Ferraro Giarita; Massai Lara; Messori LuigiCinellu Maria AgostinaMerlino Antonello

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Structural evidences for a secondary gold binding site in the hydrophobic box of lysozyme

机译：Structural evidences for a secondary gold binding site in the hydrophobic box of lysozyme

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A new crystal structure is reported here for the adduct formed in the reaction between NH4 [Au(Sac)2], AuSac2, a cytotoxic homoleptic gold(I) complex with the saccharinate ligand, and the model protein hen egg white lysozyme. To produce this adduct, AuSac2 breaks down and releases both saccharinate ligands. The resulting Au(I) ions bind the protein to ND1 and NE2 atoms of His15 but also to SD atom of the zero-solvent accessible Met105 side chain, which is located in the protein hydrophobic box. The unexpected existence of this secondary gold(I) binding site is confirmed by spectroscopic and spectrometric measurements in solution.

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《BioMetals: An International Journal on the Role of Metal Ions in Biology, Biochemistry and Medicine》 |2015年第4期|745-754|共10页
作者
Ferraro Giarita; Massai Lara; Messori LuigiCinellu Maria AgostinaMerlino Antonello;
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作者单位

Univ Florence, Dept Chem, I-50059 Florence, Italy;

Univ Sassari, Dept Chem & Pharm, I-07100 Sassari, Italy;

Univ Naples Federico II, Dept Chem Sci, I-80126 Naples, ItalyCNR Inst Biostruct & Bioimages, I-80100 Naples, Italy;

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正文语种英语
中图分类生物科学;
关键词
Gold-based drugs; Metallodrugs; Protein-ligand interactions;

Structural evidences for a secondary gold binding site in the hydrophobic box of lysozyme

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