An Electrochemical Acetylcholinesterase Biosensor Based on Fluorene(bisthiophene) Comprising Polymer for Paraoxon Detection

Huzeyfe Celik; Saniye Soylemez

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An Electrochemical Acetylcholinesterase Biosensor Based on Fluorene(bisthiophene) Comprising Polymer for Paraoxon Detection

机译：An Electrochemical Acetylcholinesterase Biosensor Based on Fluorene(bisthiophene) Comprising Polymer for Paraoxon Detection

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Abstract In this study, a novel conductive polymer comprising biosensor based on poly‐2,2′‐(9,9‐dioctyl‐9 h‐fluorene‐2,7‐diyl)bistiophene (Poly(BT)) and acetylcholinesterase (AChE) was reported for the determination of paraoxon. This practical biosensor allowed to catalyze electrochemical oxidation of acetylthiocholine (+0.6 V vs. Ag reference). The detection range for acetylcholine chloride (AThCl) with Poly(BT)/AChE was found to be 0.025–4 mM. In pesticide analysis, wide linear ranges from 0.5 to 1 μg/L and 1 to 14 μg/L, and a low detection limit of 0.033 μg/L were estimated. Under optimum operating conditions, the developed biosensor was used for pesticide detection in milk and tap water samples, effectively.

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《Electroanalysis》 |2023年第3期|n/a-n/a|共11页
作者
Huzeyfe Celik; Saniye Soylemez;
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作者单位

Ordu University;

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原文格式 PDF
正文语种英语
中图分类电化学、电解、磁化学;
关键词
Electrochemical Biosensor; Conjugated Polymer; Acetylcholinesterase; Enzyme Immobilization; Organophosphorus Pesticides;

An Electrochemical Acetylcholinesterase Biosensor Based on Fluorene(bisthiophene) Comprising Polymer for Paraoxon Detection

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