...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>journal of cellular physiology >Immunocytochemical localization of annexin V (CaBP33), a Ca2+‐dependent phospholipid‐and membrane‐binding protein, in the rat nervous system and skeletal muscles and in the porcine heart
【24h】

Immunocytochemical localization of annexin V (CaBP33), a Ca2+‐dependent phospholipid‐and membrane‐binding protein, in the rat nervous system and skeletal muscles and in the porcine heart

机译：Immunocytochemical localization of annexin V (CaBP33), a Ca2+‐dependent phospholipid‐and membrane‐binding protein, in the rat nervous system and skeletal muscles and in the porcine heart

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相关主题

摘要

AbstractWe investigated the ultrastructural localization of annexin V a Ca2+‐dependent phospholipid‐ and membrane‐binding protein in the nervous system, heart, and skeletal muscles. The results indicate that in the cerebellum the protein is restricted to glial cells, where it is found diffusely in the cytoplasm as well as associated with plasma membranes. Bergmann glial cell bodies and processes and astrocytes in the cerebellar cortex and oligodendrocytes in the cerebellar white matter displayed an intense immune reaction product. In sciatic nerves, the protein was exclusively found in Schwann cells with a subcellular localization similar to that seen in glial cells in the cerebellum. Pituicytes in the neurohypophysis were intensely immunostained, whereas axons were not. In the heart, annexin V was restricted to the sarcolemma, transverse tubules, and intercalated discs. In skeletal muscles the protein was localized to the sarcolemma and transverse tubules. No evidence for the presence of the protein in the sarcoplasm or in association with mitochondria, the sarcoplasmic reticulum, or contractile elements was obtained. The observation that plasma membranes in cells expressing annexin V have the protein associated with them is in agreement with previous data on Ca2+‐dependent binding of the protein to brain and heart membranes, and on existence of both EGTA‐ and Triton X‐100‐extractable and resistant fractions of annexin V in these membranes. The present data support the hypothesis that annexin V might be involved in membrane trafficking and suggest a role for this protein in the regulation of cytoplasmic activities in glial cells. © 1992 W

著录项

获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号