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>Interleukin 1 stimulates prostaglandin synthesis and cyclic AMP accumulation in Swiss 3T3 fibroblasts: Interactions between two second messenger systems
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Interleukin 1 stimulates prostaglandin synthesis and cyclic AMP accumulation in Swiss 3T3 fibroblasts: Interactions between two second messenger systems
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机译：Interleukin 1 stimulates prostaglandin synthesis and cyclic AMP accumulation in Swiss 3T3 fibroblasts: Interactions between two second messenger systems
AbstractBiochemical events elicited by interleukin 1 (IL–1) were studied in Swiss 3T3 fibroblasts. One hour after its addition, IL–1 stimulated synthesis of prostaglandin E2(PGE2), which continued to accumulate for 4 days. IL–1 also stimulated cAMP accumulation. Indomethacin blocked cAMP accumulation in response to IL–1, suggesting that PG2was responsible for the increase. Addition of exogneous PGE2to indomethacin‐treated cells restored cAMP accumulation. IL–1 enhanced thymidine incorporation, and indomethacin attenuated responses to lower concentrations. Thus, PGE2appeared to play a role in the ability of low concentrations of IL–1 to stimulate thymidine incorporation. PGE2augmented thymidine incorporation by increasing cAMP accumulation because in the presence of indomethacin addition of exogenous cAMP enhanced thymidine incorporation in response to low concentrations of IL–1. Elevated cAMP further stimulated PGE2synthesis. Thus, PGE2and cAMP interacted to potentiate their mutual accumulation. In summary, IL–1 stimulates PGE2synthesis. PGE2, in turn, stimulates cAMP accumulation which potentiates IL–1–stimulated PGE2synthesis and th
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