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>Physical localization of yeastCYS3, a gene whose product resembles the rat γ‐cystathionase andEscherichia colicystathionine γ‐synthase enzymes
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Physical localization of yeastCYS3, a gene whose product resembles the rat γ‐cystathionase andEscherichia colicystathionine γ‐synthase enzymes
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机译：Physical localization of yeastCYS3, a gene whose product resembles the rat γ‐cystathionase andEscherichia colicystathionine γ‐synthase enzymes
AbstractWe have cloned, sequenced and physically mapped theCYS3gene ofSaccharomyces cerevisiae. This gene can complement thecys3–1allele, and disruptions at this locus lead to cysteine auxotrophy. The predictedCYS3product is closely related (46% identical) to the rat cystathionine γ‐lyase (Ericksonet al., 1990), but differs in lacking cysteine residues. These results provide further evidence that the S288C strain of yeast resembles mammals in synthesizing cysteine solely via atrans‐sulfuration pathway. TheCYS3product was found to have strong homology to three other enzymes involved in cysteine metabolism: theEscherichia coli metBandmetCproducts and theS. cerevisiaeMET25 gene product. Thetrans‐sulfuration enzymes appears to form a diverged family and carry out related functions from bacteria to
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